真菌毒素免疫分析中新型检测元件的研究进展

Research progress in novel materials for mycotoxin immunoassay

真菌毒素免疫分析是基于抗体与真菌毒素间的相互作用。虽然具有高亲和力及特异性的抗体制备技术已非常成熟,但代替传统抗体或真菌毒素的新型检测元件越来越受到人们的关注。这些元件分为以重组抗体片段为代表的重组类检测元件,如单链抗体,以化学法人工合成的合成类检测元件,如分子印迹聚合物,及其他用于取代真菌毒素标准品的检测元件,如抗独特型抗体。本文重点综述了新型真菌毒素检测元件的研究进展,及其在真菌毒素免疫分析中的应用前景。

Mycotoxin immunoassays depend on interactions between antibodies and mycotoxins. Although intact antibodies with high affinity and specificity were well developed,novel materials instead of conventional antibodies or mycotoxins for immunoassays attracted more and more attention. The alternatives can be classified into those that are represented by recombinant antibodies and their fragments such as single chain variable fragments,and those that are obtained by chemical synthesis such as molecularly imprinted polymers,and those that are used to replace mycotoxin standards such as anti- idiotypic antibodies.Recent developments of novel materials and their potential for application in mycotoxin immunoassays were reviewed.