狗脊饮片多成分的1H—NMR分析

Multi-component of Cibotium baronetz decoction pieces via 1H-NMR spectroscopic analysis

目的:采用1H-NMR技术从多成分角度分析30批市售狗脊饮片的质量差异,建立狗脊饮片质量控制的新方法。方法:采用主成分分析和偏最小二乘法对600 MHz核磁谱仪测定的30批市售狗脊饮片1H-NMR谱图进行模式分析,揭示市售不同质地狗脊饮片内在成分上的差异。结果:狗脊饮片的质地与其多种成分含量密切相关。不同质地狗脊饮片成分差异主要表现为糖类、酚酸类、甾醇类及蕨素类成分的含量差异。狗脊饮片1H-NMR谱芳香区的酚酸类成分吸收信号可以作为评价狗脊饮片质量的指标。结论:1H-NMR技术与多元统计分析法结合能从多种成分角度提取狗脊饮片质量差异的成分信息,可以作为狗脊饮片的质量控制方法。

Objective： 1H-NMR technology was carried out to investigate the chemical difference between 30 batches of Ciboti- um baronetz decoction pieces and look for new method for quality control of C. baronetz decoction pieces. Method： Six hundreds MHz 1H-NMR spectroscopy and principle component analysis （PCA） were used to discriminate between 30 batches of commercially availa- ble cibotium samples based on muhi-component metabolite profiles. Result： Saccharide is the principle component of C. baronetz de- coction pieces, and steroid and triterpene were the discriminately chemical component. Protocatechuic acid, protocatechuic aldehyde, cibotiumbaroside A, cibotiumbaroside B and 4-O-caffeoyl-D-glucoside could be used as the marker for controlling the quality of com- mercial C. baronetz decoction pieces. Conclusion： Pattern-recognition techniques applied to proton nuclear magnetic resonance （ 1 H- NMR） spectra of 80% methanol extraction of C. baronetz could correctly discriminate not only the quality, but also the chemical com- ponent for batches of commercial C. baronetz decoction pieces.