高效毛细管电泳测定乳制品中的酪蛋白糖巨肽

Determination of caseinomacropeptide in dairy products by high performance capillary electrophoresis

建立了高效毛细管电泳(HPCE)分离测定乳制品中酪蛋白糖巨肽(CMP)的新方法。研究了影响CMP与杂质峰分离的因素,如分离缓冲溶液的种类、浓度、pH值等。结果表明,CMP校正峰面积与质量浓度在60～4 000 mg/L范围内具有良好线性关系,线性相关系数为0.9993,检出限(S/N=3)为20 mg/L,定量限为60 mg/L。该方法简单快速,适用于添加乳清蛋白粉的各种乳制品中酪蛋白糖巨肽的常规检测。

A new method for the quantitative determination of caseinomacropeptide （CMP） by high performance capillary electrophoresis （HPCE） was established. The detection wavelength was set at 214 nm. The key factors that affect the separation such as the concentration of additives, the concentration and pH of the running and sample buffer, etc. were investigated in detail. A linear relationship was established for the CMP concentration in the range from 60 mg/L to 4 000 mg/L with a linear correlation coeflacient （r=0.9993）. The limit of detection （LOD, S/N=3） and quantitation （LOQ, S/N=10） were 20 mg/L and 60 rag/L, respectively. The method was simple and rapid for the routine determination of CMP in different kinds of dairy products adulterated with whey protein.