甘肃棘豆对SD大鼠组织损伤的病理学

Pathological study on tissue injury of Oxytropis kansuensis Bunge in SD rats

为探讨不同剂量甘肃棘豆对大鼠不同组织的病理学损伤,试验将16只SD大鼠随机分为4组,每组4只,分别标记为对照组、试验Ⅰ～Ⅲ组,分笼饲养,自由采食和饮水。对照组饲喂全价饲料,试验Ⅰ～Ⅲ组分别饲喂含15%,30%,45%甘肃棘豆的混合日粮,期间记录各组大鼠体质量及临床症状,染毒结束后采集脑、肝脏、肾脏、脾脏,制作石蜡切片观察其病理组织学变化。结果显示,甘肃棘豆能引起SD大鼠脑组织部分神经细胞肿胀、树突变短或消失、胞浆空泡化、且HE染色变淡;小脑浦肯野细胞、脑干网状结构、肾脏、肝脏、脾脏等多种组织出现以空泡变性为特征的病理变化。结果表明,甘肃棘豆对SD大鼠生长发育具有明显抑制作用,且能引起机体各组织器官广泛性病理损伤。

In order to ob rand each omly divided into four ,all the animals were a feeded weight sues and pat The resuhs basic linical hology g 11 diet with symptom the lesions induced by Oxylropis kansuensis Bunge, 16 SD rats were roups（experimental group , Ⅰ,Ⅱ,Ⅲand control group） of 4 animals owded to forage and drink freely. The rats were penned as groups and 0%, 15%, 30% and 45% O. kansuensis Bunge, respectively. Body changes of heart,liv showed that the interspaces d midbrain etc, which was er,spl round een,lung and kidney were observed after poisoning. ecame lightly dyed with HE stainin Bunge exposure presents a significant inhibilory effect on tile growth larger in the thalamus,hypo- g compared with the normal spleen displayed pathological ed that Oaytropis kalzsuensis and development of SD rats, and can cause extensive pathological damages in tissues and organs of SD rats.