丙泊酚对幼小鼠自主活动和学习记忆行为的影响

Effects of propofol on spontaneous motor activity and learning behaviors in juvenile mice

目的观察丙泊酚(Prof)对幼小鼠自主活动和学习记忆行为的影响,探讨其与GABAA受体的关系。方法 300只9～10日龄小鼠分为3大组,分别进行自主活动实验、避暗实验和跳台实验,各组小鼠ip Prof或NS 10 min后icv荷包牡丹碱(Bic)或人工脑脊液(aCSF)。测试最后一次给药后15、30、45和60 min小鼠5 min内活动次数;跳台仪和避暗仪记录小鼠步入、跳下的潜伏期和错误次数。结果与NS组比,Prof可减少小鼠自主活动次数;给Prof 24h后小鼠步入和跳下潜伏期缩短、错误次数增加(P<0.05)。Bic可增加Prof小鼠自主活动次数,延长其跳下和步入潜伏期,减少错误次数(P<0.05)。结论 Prof可减少幼小鼠自主活动次数,损害学习记忆能力,GABAA受体是其重要靶位。

Aim To investigate the effects of propofol（prof） on spontaneous motor activity and learning behaviors and to explore their relationships with GABAA receptor in juvenile mice.Methods Three hundred mice were divided into three main groups for three experiments.Each group was subdivided into ten subgroups：normal control,Prof 25,50,100 mg·kg-1,Bic 0.05,0.1,0.2 μg,and Prof 100 mg·kg-1＋Bic 0.05,0.1,0.2 μg.Prof was ip given 10 min before Bic icv given.Locomotor activities in 15,30,45 and 60 min after given Bic were recorded and the latency and error number of step down and step through test in 1d and 2d were detected.Results Compared with NS group,Prof decreased the locomotor activities,and the latency and increased the error number of step down and step through tests in juvenile mice.Bic didn′t affect the locomotor activities,the latency and the error number of step down and step through tests in juvenile mice.But Bic decreased the effect of Prof on locomotor activities,the latency and error number of step down and step through tests in juvenile mice.Conclusion Propofol can decrease the locomotor activities and restrain learning and memory function in juvenile mice,and GABAA receptor may be involved in the effects.