黄芪甲苷对脂肪源性干细胞体外生物学行为的影响

Effects of Astragaloside IV on biological behavior of adipose tissue-derived stem cells

目的探讨黄芪甲苷(Astragaloside IV,Ast)对体外培养人脂肪源性间充质干细胞(human adipose-derived mesen-chymal stem cells,hADSCs)生物学行为的影响。方法应用CCK8和PCNA法检测不同浓度和时间Ast孵育对hADSCs增殖情况的影响,选取出Ast最适干预条件并对hADSCs进行培养,观察药物干预后细胞形态、表面标志物、成骨及成脂分化潜能。结果 CCK8和PCNA结果均显示,Ast 20 mg.L-1干预48 h后细胞增殖最为明显,进一步选取Ast20 mg.L-1干预hADSCs 48 h,与对照组相比,两组细胞CD29、CD44、CD105的阳性率均为96%-99%;两组细胞成骨诱导与成脂诱导结果也无差异。结论 Ast可促进ADSCs增殖,以20 mg.L-1、孵育48 h为最适干预条件,且该条件干预后并不影响hADSCs细胞形态,表面标志物水平及多向分化能力。

Aim Human adipose-derived mesenchymal stem cells（hADSCs） were cultured with Astragaloside IV（Ast） in vitro so as to approach the influence of biological behaviour.Methods Cell proliferation was determined by Cell-Counting Kit-8（CCK8） and proliferating cell nuclear antigen（PCNA）.The most suitable intervention conditions were selected and then cells were identified as MSCs by adherence to plastic,spindle shaped morphology,specific surface markers,differentiation abilities into osteoblasts,adipocytes and chondroblasts in vitro under appropriate conditions.Results hADSCs cultured with 20 mg·L^-1 Ast for 48 hours had the most significant effect on proliferation of cells,which showed typical fibroblast-like phenotype and expressed a high level of typical MSCs markers CD29,CD44,CD105（96% to 99%）.They could also differentiate into osteoblasts and adipocytes in vitro under appropriate conditions.Conclusions The Ast（20 mg·L^-1 for 48 hours） could obviously promote the proliferation of hADSCs and has no influence on the morphology,specific surface markers and differentiation abilities of hADSCs.