摘要
目的探究绿原酸及其同分异构体对人血浆阿司匹林酯酶活性的影响。方法以高效液相色谱法测定阿司匹林酯酶体外培养体系中水杨酸(SA)和阿司匹林(ASA)的浓度,以CSA/(CASA+CSA)反映阿司匹林酯酶的活性。通过空白对照组和添加单体成分的实验组间阿司匹林酯酶活性的变化来分析各成分对阿司匹林酯酶活性的影响。结果实验组与空白对照组之间差异没有显著性。结论在本实验浓度下,绿原酸及其同分异构体对酯酶影响不明显,为临床同时使用阿司匹林与含有该类化合物的中药或中药制剂提供理论依据。
Aim To investigate the effects of chlorogenic acid and its isomers on the activity of aspirin esterase in human plasma.Methods The concentrations of aspirin（ASA） and its metabolite salicylic acid（SA） in the culture in vitro were determined by HPLC.The ratio of CSA/（CASA＋CSA） was used as the activity of aspirin esterase.The effects of chlorogenic acid and its isomers on aspirin esterase were evaluated by comparing the activities of aspirin esterase in human plasma between blank control and experimental group.Results The results suggested that there were no significant differences between the blank control and experimental group.Conclusions Chlorogenic acid and its isomers had no significant effect on the activity of aspirin esterase at the experimental concentrations.As a few herbs and herbal preparations contained experimental compounds,the conclusion provides practical information for clinical practice for herbs（or herbal preparations） and aspirin used concurrently.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2013年第2期225-228,共4页
Chinese Pharmacological Bulletin
基金
科技部重大新药创制科技重大专项(No 2012ZX09303009-002)
江苏省中医药领军人才(No LJ200906)
江苏高校优势学科建设工程资助项目(2010)
