淡竹叶黄酮对大鼠心肌缺血/再灌注损伤的保护作用

Protective effects of total flavones from Lophatherum gracile on myocardial ischemia-reperfusion injury in rats

目的研究淡竹叶总黄酮(TFLG)对大鼠心肌缺血/再灌注损伤的作用及其机制。方法采用结扎大鼠左冠状动脉前降支法,建立心肌缺血/再灌注损伤模型。测定血清乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、一氧化氮(NO)含量。结果 TFLG 50、100 mg.kg-1可抑制大鼠心肌中LDH及CK的漏出,降低血清和心肌组织中LDH与CK活性,降低MDA含量,提高SOD、GSH-PX和NO浓度。TFLG 100 mg.kg-1可抑制NF-κB和TNF-α蛋白的表达,下调caspase-3蛋白表达。结论 TFLG对心肌缺血/再灌注损伤有一定的保护作用,其作用机制可能与抗自由基、抑制炎症反应和减少细胞凋亡有关。

Aim To study the protective effect of total flavones from Lophatherum gracile（TFLG） on myocardial ischemia-reperfusion injury in rats.Methods The ischemic model was made by occluding the anterior descending of the left artery（LAD） in rats.The activity of lactate dehydrogenase（LDH）,creatine kinase（CK）,levels of the superoxide dismutase（SOD）,maleic dialdehyde（MDA）,glutathione peroxidase（GSH-PX） and nitric oxide（NO） in serum and myocardial tissue were measured.Results TFLG（50 and 100 mg·kg^-1） obviously reduced the activity of CK and LDH,decreased the level of MDA and increased the content of SOD,GSH-PX and NO.TFLG（100 mg·kg^-1） inhibited the expression of NF-κB and TNF-α and reduced the expression of caspase-3 protein.Conclusion TFLG has certain protective effect on myocardial ischemia-reperfusion injury in rats via attenuating oxygen free radical,inhibiting inflammatory reaction and reducing apoptosis.