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猪流行性腹泻病毒的基因结构及其诊断技术 被引量:6

Progress in research of genetic characteristics of porcine epidemic diarrhea virus and diagnostic methods for porcine epidemic diarrhea
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摘要 猪流行性腹泻(porcine epidemic diarrhea,PED)是由猪流行性腹泻病毒(PED virus,PEDV)引起的猪的一种高度接触性肠道传染病,以呕吐、水样腹泻和脱水为特征,在亚洲地区尤其是我国养猪场的感染非常严重.猪流行性腹泻的流行病学特征、临床症状以及病理变化与猪传染性胃肠炎(transmissible gastroenteritis of swine,TGE)非常相似,确诊需要借助实验室检测.近年来,随着分子生物学技术的发展和人们对PEDV基因结构及功能认识的深入,PED的诊断技术也取得了很大进展.本文主要对PEDV的基因结构、功能以及诊断技术方面取得的新进展进行综述,以期为PEDV基因的深入研究以及建立特异、快速、简便的诊断方法提供新的思路. Porcine epidemic diarrhea(PED),caused by PED virus(PEDV),is a devastating contagious viral swine disease that is characterized by acute enteritis and lethal watery diarrhea.In Asia(such as Korea,Japan,and China),PEDV causes a very high mortality in suckling piglets.Some affected farms lost 100% of newborn piglets.PED can hardly be distinguished from transmissible gastroenteritis clinically and histopathologically.Identification of the causal agent is a basic prerequisite both for introduction of immunoprophylactic measures and for evaluation of prevention measures.Therefore,rapid differential diagnosis of PED and TGE is important.The purpose of the current review is to describe the molecular and genetic characteristics of PEDV,and discuss the diagnosis methods for PED.
出处 《世界华人消化杂志》 CAS 北大核心 2013年第1期54-59,共6页 World Chinese Journal of Digestology
基金 国家自然科学基金资助项目 No.31272593 河北省高等学校科学研究计划基金资助项目 No.2011180~~
关键词 猪流行性腹泻病毒 基因结构 诊断方法 Porcine epidemic diarrhea Genetic structure Diagnostic methods
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  • 1赵青,钟土木,毛方军,孙渡江.早期断奶仔猪腹泻病药物防治试验[J].浙江农业学报,2005,17(2):90-93. 被引量:9
  • 2干小仙,王越,杨发柱,张莉莉,Praphathip Eamsobhana.金标法检测广州管圆线虫特异性IgG抗体的研究[J].中国人兽共患病学报,2007,23(4):345-347. 被引量:7
  • 3PENSAERT M B, DEBOUCK P. A new coronavirus-like particle associated with diarrhea in swine[J]. Arch Virol, 1978, 58: 243-247.
  • 4Holmes K. Coronaviruses [ M]//Fields Virology. KNIPE D M, HOWLEY P M, GRIFFIN D E. 4th ed. Philadelphia: Lippincott Williams & Wilkins,2001: 1187-1197.
  • 5LIB X, GE J W, LI Y J. Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus[J]. Virology, 2007, 365: 166-172.
  • 6SMITH G P. Affinity selection[M/OL]. [2005b 2006- 05-151. http://www, biosci, missouri. edu/smithgp/ PhageDisplayWebsite/AffinitySelection. doe.
  • 7GODET M, GROSCLAUDE J, DELMAS B, et al. Major receptor-binding and neutralization determinants are located within the same domain of the trans- missible gastroenteritis virus (coronavirus) spike protein[J]. J Virol, 1994, 68: 8008-8016.
  • 8KUBOTA S, SASAKI O, AMIMOTO K, et al. Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus[J]. J Vet IVied Sci, 1999, 61: 827-830.
  • 9BONAVIA A, ZELUS B D, WENTWORTH D E, et al. Identification of a receptor-binding domain of the spike glycoprotein of human eoronavirus HCoV-229E[J]. J Virol, 2003, 77: 2530-2538.
  • 10BABCOCK G J, ESSHAKI D J, THOMAS W D, et al. Amino acids 270 to 510 of the severe acute respiratory syndrome coronavirus spike protein are required for interaction with receptor[J]. J Virol, 2004, 78: 4552-4560.

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