海水中丙烯酸的高效液相色谱法建立及应用

Determination of acrylic acid in seawater by high performance liquid chromatography and its application

建立了一种测定海水介质中丙烯酸的高效液相色谱法。采用耐纯水相和较低pH的AgilentSB-Aq-C18柱(100mm×4.6mm i.d.,5μm),0.35%磷酸溶液(pH=2)为流动相,使用紫外检测器,检测波长为210nm,外标法定量测定。丙烯酸的保留时间在14.2～14.9min,方法的检出限为4nmol/dm3(S/N=3),在0.01～10μmol/dm3的范围内均有良好的线性关系,相关系数可达0.999 6,加标回收率为95.4%～98.1%,相对标准偏差为1.3%～1.6%(1.04～2.32μmol/dm3)。采用0.2μm滤膜重力过滤冷藏的办法来保存海水丙烯酸样品。检测出2011年5月份青岛近海海水中丙烯酸的平均浓度为(0.101±0.069)μmol/dm3;海洋微藻球形棕囊藻在整个生长周期内培养体系中丙烯酸的浓度为0.339～2.219μmol/dm3。

A reversed phase high performance liquid chromatographic method was developed to determine dissolved acrylic acid in seawater. Using the Agilent SB-Aq-C18 column （100 ram）〈 4.6 mm i.d., 5 /xm） and 0.35%. H3PO4-water （pH= 2） as the mobile phase, the column eluate was monitored by ultraviolet detector. The wave- length of UV detector was 210 nm, and an external standard method was adopted. The detection limit of the method was 4 nmol/dma （S/N=3）. The peak area is linear with acrylic acid concentration in the range of 0.01- 10/,mol/dma , with a correlation coefficient of 0. 999 6. The average recovery was 95.4 %- 98. 1 % and RSD was 1.3%- 1.6%. Samples for acrylic acid could be stored in the dark at 4℃ after gravity filter through 0.2 μmWhatman membrane. The highly sensitive and accurate analytical method has been successfully applied to the determination of acrylic acid in natural seawater and algal culture.