C57BL/6小鼠食管鳞状细胞癌早期病变的形态学改变

Morphology of early lesions of esophageal squamous cell carcinoma in C57BL/6 mice

目的:了解食管癌发生过程中的炎症改变.方法:采用100?g/mL的4NQO通过饮水作用于C57BL/6小鼠,分别通过食管拉网脱落细胞法、碘染色法及病理组织学观察第12、16、20、24周等时间段的C57BL/6小鼠食管鳞状细胞癌建模病理的改变.结果:食管拉网脱落细胞法、碘染色法均未观察到小鼠早期食管病变,在实验第12周,纵向解剖食管,通过病理组织学观察到食管上皮不典型增生,第16、20、24周分别观察到原位癌、浸润性鳞癌的发生,在整个肿瘤的发生、发展过程中伴随炎症细胞浸润.结论:食管拉网脱落细胞法、碘染色法不适用于C57BL/6小鼠食管鳞癌建模形态学观察,只能通过病理组织学才能够在不同的时间段观察到食管癌的发生、发展及其炎症改变过程.

To dynamically observe the morphology of early lesions of esophageal squamous cell carcinoma （ESCC） in C57BL/6 mice. METHODS： ESCC was induced in C57BL/6 mice by giving drinking water containing 100 μg/mL of 4-nitroquinoline-l-oxide. During the experiment period, the morphology of early lesions of ESCC was observed by esophageal exfoliated cytology, iodine staining, observation with naked eyes, and histological assessment at weeks 12, 16, 20 and 24 after induction of ESCC.RESULTS： Early esophageal lesions could notbe observed by esophageal exfoliated cytology or iodine staining. Esophageal epithelial dysplasia was observed by longitudinally dissecting the esophagus at week 12. In situ carcinoma and invasive cancer were observed at weeks 16, 20, and 24. Inflammation existed during the entire process of the development of ESCC. CONCLUSION： Histopathological techniques, not esophageal exfoliated cytology or iodine staining, are suitable for observing the morphol- ogy of early ESCC lesions in C57BL/6 mice.