培菲康对高脂饮食诱导脂肪肝病大鼠肠黏膜屏障的保护作用

Effect of Bifidobacterium triple viable capsule on intestinal mucosal barrier function in rats with fatty liver induced by a high-fat diet

目的:观察培菲康对高脂饮食诱导大鼠非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)肠道菌群的干预作用及紧密连接蛋白occludin、白介素(interleukin,IL)-17在回肠黏膜上表达的影响.方法:♂SD大鼠34只分为高脂饮食组(Ⅰ组,n=12),高脂饮食+培菲康干预组(Ⅱ组,n=12),普通饮食组(Ⅲ组,n=10).Ⅱ组予培菲康灌胃,余下两组分别给予相同剂量生理盐水灌胃.于喂养17wk处死各组大鼠,肝脏组织病理表现提示NAFLD模型成功建立.采用16S RNA方法检测3组大鼠肠道3种主要细菌数量;采用透射电镜观察回肠组织超微结构;免疫组织化学方法观察回肠黏膜occludin蛋白及IL-17量的表达变化.结果:(1)Ⅰ组、Ⅱ组较Ⅲ组:肠道3种细菌数量及紧密连接蛋白occludin表达差异均有显著性(P<0.05);(2)Ⅰ组较Ⅱ组:乳酸杆菌数量、蛋白occludin表达量差异无显著性;(3)IL-17表达在3组间差异有显著性(36.289±30.540vs9.646±14.530vs2.609±5.850);此外,透射电镜下观察到Ⅰ组大鼠肠上皮细胞水肿,绒毛排列紊乱,线粒体肿胀、疏松化明显,并出现坏死细胞,而经培菲康干预后的大鼠肠上皮损伤减轻.结论:培菲康对高脂饮食诱导大鼠NAFLD有一定预防作用,其作用可能与保护高脂饮食引起的肠黏膜屏障损伤有关.

AIM： To investigate the potential preventive effect of Bifidobacterium triple viable capsule （Birico） on intestinal microflora and the expression of IL-17 and occludin proteins in ileum mucosa in rats with non-alcoholic fatty liver disease NAFLD induced by a high-fat diet. METHODS： Thirty-four male Sprague-Dawley rats were divided into three groups and were given a high-fat diet （group I, n = 12）, a high-fat diet and Bifico （group II, n - 12）, and an ordinary diet （group IlI, n = 10）. Rats of group lI were given Bifico gavage, while the other two groups were given the same amount of normal saline. Seventeen weeks later, the rats were killed for having a liver biopsy to confirm whether NAFLD was successfully induced. The 16S RNA method was used to detect the numbers of three main types of bacteria in the intestinal tract. The ultrastructure of ileum tissue was observed by electron microscopy, and the expression of occludin and IL-17 proteins was detected by immunohistochemistry. RESULTS： Compared to group llI, there was a significant increase in the number of Escherichia coli, Lactobacillus, and Bifidobacteria, and expression of occludin in groups I and II （all P 〈 0.05）. There was no significant difference in the number of LactobaciUus and expression of occludin between groups I and lI. The expression of IL-17 differed significantly between the three groups （all P 〈 0.05）. Electron microscopy indicated that intestinal epithelial cells in rats given a high-fat diet showed a series of pathological injuries （e.g., hydropsia and necrosis）, and the use of Bifico could relieve intestinal epithelial injury. CONCLUSION： Bifico exerts a preventive effect on non-alcoholic fatty liver disease induced by a high-fat in rats possibly by protecting the intestinal mucosal barrier.