TGF-β1、TIEG1和Stathmin蛋白在食管鳞状细胞癌组织中的表达及临床意义

Clinical signif icance of expression of TGF-β1,TIEG1 and stathmin proteins in esophageal squamous cell carcinoma

目的:探讨转化生长因子-β1(transforming growth factor-β1,TGF-β1)、早期基因1(transforming growth factor β-inducible earlygene1,TIEG1)和Stathmin蛋白在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及临床意义.方法:应用免疫组织化学法检测62例ESCC、31例癌旁不典型增生组织及62例正常食管黏膜组织中TGF-β1、TIEG1和Stathmin蛋白的表达情况.结果:正常食管黏膜组织中TGF-β1和TIEG1蛋白表达的阳性率显著高于非典型增生组织和食管癌组织[TGF-β1:62(100.0%)vs22(71.0%),41(66.1%),P<0.05].食管鳞癌组织和癌旁不典型增生组织中也检测到Stathmin蛋白表达,但较TGF-β1和TIEG1表达范围窄.正常食管黏膜组织中未检测到Stathmin的表达.进一步统计分析表明Stathmin与TGF-β1、TIEG1在食管鳞癌的表达呈负相关[TGF-β1:r=-0.609,r=-0.459;TIEG1:P<0.05);高表达Stathmin的病例中TGF-β1和TIEG1低表达或不表达.统计结果还表明TGF-β1、TIEG1和Stathmin蛋白表达与癌的临床分级及淋巴结转移密切相关(P<0.05).结论:TGF-β1和TIEG1蛋白可能结合Stathmin结合位点,负性调节Stathmin蛋白,抑制食管癌的转移.

AIM：To analyze the clinical significance of expression of transforming growth factor-β1（TGF-β1）,transforming growth factor β-inducible early gene 1（TIEG1） and stathmin in esophageal squamous cell carcinoma（ESCC）.METHODS：Immunohistochemistry was used to detect the expression of TGF-β1,TIEG1 and stathmin in 62 cases of ESCC,31 cases of tumoradjacent atypical hyperplasia epithelium and 62 cases of normal esophageal epithelium.RESULTS：The positive rates of TGF-β1 and TIEG1 proteins in normal esophageal epithelium were significantly higher than those in tumoradjacent atypical hyperplasia epithelium and ESCC [TGF-β1：62（100.0） vs 22（71.0）,41（66.1）,P0.05].The expression of stathmin was also noted in ESCC and tumor-adjacent atypical hyperplasia epithelium,but its expression was not as wide as that of TGF-β1 and TIEG1.In normal esophageal epithelium,the expression of stathmin was not detected.Expression of stathmin in ESCC had a negative correlation with TGF-β1 and TIEG1 expression（r=-0.609,-0.459,both P0.05））.The expression of TGF-β1,TIEG1 and stathmin proteins was closely correlated with clinical grade and lymph node metastasis in ESCC（all P0.05）.CONCLUSION：TGF-β1 and TIEG1 may bind to stathmin,down-regulate stathmin expression and inhibit the metastasis of ESCC.