摘要
Sponges (phylum Porifera) are the phylogenetically oldest metazoa and highly efficient filter feeders.In the marine ecosystem,they are unconditionally exposed to environmental stresses.Understanding the sponge-bacteria interaction is hence of both ecological and biological significance.This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria,Escherichia coli and infectious bacteria,Vibrio spp.by measuring the 14-3-3 mRNA expression of H.perleve.Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H.perleve were cloned and sequenced.Using Reverse-transcription real-time PCR,the 14-3-3 mRNA expression of H.perleve was examined when exposed to three common bacteria in aquatic water-E.coli and two Vibrio spp.for different time and dosages.H.perleve could efficiently remove E.coli from the water column without self-infection;however Vibrio at higher dosages infected H.perleve.When H.perleve was exposed to E.coli (1.1×10 7 CFU mL-1),V.anguillarum Ⅱ (1.2×10 6 CFU mL-1) and V.alginolyticus (3.6×105 CFU mL-1) for 6h,the 14-3-3 mRNA expression in the V.anguillarum Ⅱ and V.alginolyticus groups was down-regulated by 2.67-and 2.36-fold,respectively.The 14-3-3 mRNA expression in the E.coli group was not significantly different.However,no clear trend was observed on the 14-3-3 transcript levels of H.perleve in response to different doses of V.anguillarum Ⅱ for different time.The results demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H.perleve.
Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
基金
financial supports from Chinese Academy of Sciences
"Innovation Fund"from Dalian Institute of Chemical Physics
National Hi-Tech Research and Development Program of China(2006AA09Z435)
the European Commission(Project:EVK3-CT1999-00005UVTOX)
Ph.D.Research Fund from Science and Technology Bureau of Liaoning Province of China(20091019)
Laboratory Program from Key Laboratory of Nearshore Marine Environmental Research of Liaoning Higher Education(LS2010024)
Science and Technology Program from Ocean and Fisheries Department of Liaoning Province of China(200917)
Open Laboratory Program from Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology
Ministry of Agriculture of the People's Republic of China(Open-09-13)
Public Welfare Projects from State Oceanic Administration(200805030)
