微型CT检测血清素对雌性小鼠骨皮质和骨小梁参数的影响

Effects of serotonin on cortical and trabecular bone parameters of female mice detected by Micro-CT

目的:探讨不同剂量血清素对雌性小鼠微型CT检测骨皮质和骨小梁参数的影响。方法:给予2月龄雌性C57BL/6小鼠皮下注射不同剂量(0、5、10、20、50、100 mg/kg)的血清素,3个月后通过微型CT定量分析各组小鼠第5腰椎体、股骨远端干骺端和股骨中段的相关骨参数。结果:与对照组相比,10～100 mg/kg血清素处理组小鼠骨小梁数量、骨小梁体积分数和面积分数、骨小梁矿物质密度和骨矿含量明显降低,骨小梁分离度明显升高;50～100 mg/kg血清素处理的小鼠骨皮质体积分数、骨矿含量以及骨皮质厚度明显降低。结论:血清素对小鼠骨代谢具有重要的负性调节作用。

Objective：To investigate the effects of serotonin of different doses on cortical and trabecular bone parameters of female mice.Methods：Wild-type（wt）C57BL/6 female mice with 2-month-old were injected with serotonin of different doses（0,5,10,20,50 and 100 mg/kg）subcutaneously（s.c.）daily for 3 months.Micro-CT（μCT）was used to quantitatively analyze bone parameters of the fifth lumbar bodies,distal femurs and midshafts of the femurs of each group of mice.Results：Compared to the control groups mice,the 10100 mg/kg groups had significantly reduced trabecular numbers,trabecular bone volume and surface density,and increased trabecular separation,leading to a decrease of trabecular bone mineral density and bone mineral capacity.Mice in the 50-100 mg/kg groups caused significantly decreased cortical bone mineral capacity secondary to reduced cortical thickness and cortical bone volume density.Conclusion：Serotonin has a significantly negative regulation on bone metabolism in mice.