附子-甘草配伍前后汤液中沉积物的化学组分对比研究

Comparison on chemical components in sediments of Aconiti Lateralis Radix Preparata-Glycyrrhizae Radix et Rhizoma before and after their compatibility

目的研究附子-甘草配伍前后汤液中沉积物的化学组成,以揭示附子-甘草配伍作用机制。方法采用高效液相-飞行时间质谱(HPLC-TOF-MS)对比分析附子-甘草合煎前后沉积物中6种酯型生物碱的量,借助傅里叶变换红外光谱(FTIR)及二阶光谱比较附子-甘草合煎前后沉积物的结构变化。结果附子配伍甘草合煎过程中,附子中大量酯型生物碱被甘草成分复合而发生沉积,沉积物产生的机制初步确定为生物碱中叔胺N与甘草羧酸C=O发生缔合。结论初步探析了附子-甘草合煎前后汤液中沉积物化学组分变化,有助于揭示附子-甘草配伍过程中化学组分变化及配伍作用机制。

Objective To study the chemical components in the sediments of Aconiti Lateralis Radix Praeparata （ALRP）- Glycyrrhizae Radix et Rhizoma （GRR） before and after their compatibility and to reveal the mechanism. Methods The contents of six ester alkaloids in the sediments of single ALRP and ALRP-GRR compatibility were compared simultaneously using HPLC-TOF-MS method, and the structural changes of the sediments in single ALRP and ALRP-GRR compatibility were identified using FTIR and second-derivative spectra. Results The sediments in ALRP-GRR compatibility would be produced by the association reaction between tertiary amine of alkaloids and carboxyl of compounds in GRR. Conclusion In this paper, the chemical information of the sediments in single ALRP and ALRP-GRR compatibility is preliminarily investigated, which would be beneficial to uncovering the material basis changes and compatibility mechanisms of ALRP and GRR.