党参微卫星引物筛选及群体遗传多样性研究

Microsatellite primer screening and population genetic diversity of Codonopsis pilosula

目的利用筛选的党参多态性微卫星引物进行党参群体遗传多样性研究。方法利用磁珠富集法分离党参核基因组微卫星引物,并选取党参4个野生居群48个样品进行遗传多样性分析。结果筛选出了10个能成功扩增党参样品的微卫星引物;这些引物扩增产物的等位基因数目为5～14个,观察杂合度范围为0.446～0.833,期望杂合度范围为0.697～0.895;使用4个党参近缘物种(轮叶党参、球花党参、鸡蛋参和长叶党参)进行引物通用性检测,发现有5对引物(Cpi01、Cpi04、Cpi06、Cpi07和Cpi08)能同时在4个物种中成功扩增。结论筛选出的多态性微卫星引物能用于党参的遗传多样性和群体遗传结构研究,同时也能够用于党参属其他近缘物种的群体遗传学研究。

Objective To study the genetic diversity of Codonopsis pilosula using the screened polymorphic microsatellite primers. Methods The microsatellite primers of C. pilosula were isolated based on nuclear genome by magnetic bead enrichment, and genetic diversity was examined in 48 individuals from four wild populations of C. pilosula. Results Ten microsatellite primers which could be used to amplify the sample of C. pilosula were screened and the number of alleles for the primer amplification products ranged 5--14. The ranges of observed and expected heterozygosities were 0.446--0.833 and 0.697--0.895, respectively. The transferability of these microsatellite loci was tested upon four closely related species （C. lanceolata, C. subglobosa, C. convolvulacea, and C. longifolia） of C. pilosula, among which five pairs of primers （Cpi01, Cpi04, Cpi06, Cpi07, and Cpi08） were successfully amplified. Conclusion These polymorphic microsatellite primers could be used to study the genetic diversity and population genetic structure of C. pilosula and those of other related species of Codonopsis Wall.