摘要
目的:建立离子色谱-氢化物发生原子荧光法测定尿中形态砷的方法。方法:尿样经0.45μm滤膜过滤,以(NH4)2HPO4为流动相,采用离子色谱-氢化物发生原子荧光联机测定不同形态砷的含量。结果:实验结果相关性好,线性范围宽,精密度RSD为2.60%~4.30%,方法检出限为As(Ⅲ)2.0μg/L,DMA4.0μg/L,MMA4.0μg/L,As(Ⅴ)8.0μg/L,该方法所得回收率为90.48%~102.90%。结论:方法简便、快速、无需化学预处理、干扰少、灵敏度高,有较高的实用价值。
Objective:To develop a method for the determination of arsenic species in urine by Ion chromatography-hydride Generation-atomic fluorescence spectrometry.Methods: The urine sample was filtered by 0.45 μm membrane.Using(NH4)2 HPO4 as mobile phase to explore the best ion chromatographic separation condition and the atomic fluorescence determination condition.The content of various forms of arsenic was determined by Ion chromatography-hydride Generation-atomic fluorescence spectrometry.Results: This method had good relativity and good precision(2.60%~4.30%).The detection limits of As(Ⅲ),DMA,MMA and As(Ⅴ)were 2.0 μg/L,4.0 μg/L,4.0 μg/L,8.0 μg/L,the average recoveries of samples were 90.48%~102.90%.Conclusion: The method had the advantages of convenience,speediness,high sensitivity,less interference and high practical value without chemical pretreatment.
出处
《中国卫生检验杂志》
北大核心
2013年第1期58-60,共3页
Chinese Journal of Health Laboratory Technology
关键词
离子色谱-氢化物发生原子荧光法
形态砷
尿
Ion chromatography-hydride Generation-atomic fluorescence spectrometry
Arsenic species
Urine