苏州市部分水产品分离的霍乱弧菌耐药状况及毒力基因分析

Analysis of antibiotic resistance and virulence genes of vibrio cholerae isolated from part of aquatic products in Suzhou

目的:分析苏州市部分养殖水产品霍乱弧菌分布情况,并对其进行耐药性分析和毒力基因检测。方法:对甲鱼、牛蛙及其养殖用水等样品,进行霍乱弧菌的分离、鉴定及耐药性分析和4种毒力基因的PCR检测。结果:分离到的18株霍乱弧菌标本中O139群8株,O1群4株,其余6株为非O1非O139群霍乱弧菌。经耐药性分析,对试验的12种抗生素均无100%敏感株;对多粘菌素B和红霉素的中介率高达83.3%。检测的4个毒力基因中,阳性率最高的是Zot基因。结论:分离到的18株霍乱弧菌血清型有一定的种属相关特性,中介耐药性突出,毒力基因阳性率较高。

Objective：To analyze the distribution of vibrio cholerae in some breeding aquatic products in Suzhou,to understand the antibiotic resistance and virulence genes.Methods： Vibrio cholerae was isolated from samples of soft-shelled turtles,bullfrogs and breeding water for identification.The detected vibrio cholerae strains were analyzed for antibiotic resistance.PCR method was adopted to detect 4 kinds of virulence genes.Results： Eighteen strains of vibrio cholerae were isolated,including serogroup O139（8 strains）,serogroup O1（4 strains）,non-O1 and non-O139（6 strains）.The antibiotic resistance result indicated that none of the 18 vibrio cholerae strains were 100% sensitive to 12 kinds of tested antibiotics;while the antibiotic intermediate resistance rate to polymyxin B and erythromycin was up to 83.3%.Of four virulence genes,zonula occludens toxin gene was the most prevalent.Conclusion： There was certain species-dependent characteristics in 18 strains of vibrio cholerae by serological typing,prominent antibiotic intermediary resistance and high positive rate of virulence gene were also the features of the 18 vibrio cholerae strains.