Sysmex UF-1000i尿有形成分分析仪的性能评价

Evaluation of performance of sysmex UF-1000i urine formed elements analyzer

目的:评价Sysmex UF-1000i尿液有形成分分析仪的检测性能。方法:分别检测WBC、RBC、上皮细胞(EC)、管型(CAST)及细菌(BACT)等参数的精密度、线性范围和携带污染率。应用Fuchs-Rosenthal计数板和UF-1000i分别对200例尿液样本WBC、RBC、EC、CAST进行定量计数并进行相关和线性回归分析。结果:WBC、RBC、EC、CAST和BACT的批内精密度、批间精密度、线性范围和携带污染率均在仪器要求范围内。应用Pearson相关分析,UF-1000i与定量显微镜计数检测WBC、RBC、EC、CAST的相关系数(r)分别为0.997、0.997、0.949、0.734。应用线性回归分析,UF-1000i与定量显微镜计数检测WBC、RBC、EC、CAST的决定系数(R2)分别为0.994、0.994、0.900、0.553。结论:Sysmex UF-1000i尿液有形成分分析仪各项性能均符合仪器要求范围,并能较为准确定量WBC、RBC和EC,可作为尿液有形成分分析筛查工具。

Objective：To evaluate the performance of Sysmex UF-1000i urine formed elements analyzer.Methods： A total of 200 urine samples were collected to test the precision,linear range and contamination rate of white blood cell（WBC）,red blood cell（RBC）,epithelial cells（EC）,CAST and bacteria（BACT） by UF-1000i analyzer.The microscopic count was carried out by Fuchs-Rosenthal counting plate.The results of WBC,RBC,EC and CAST were analyzed with pearson statistics and linear regression analysis.Results： Precision,linearity and contamination rate of WBC,RBC,EC,CAST and BACT were all in the allowable range supplied by factory authorities.Pearson correlation analysis showed the correlation coefficients of UF-1000i test and quantitative microscopy test were 0.997 for WBC and RBC,0.949 for EC and 0.734 for CAST.The linear regression analysis showed the determination coefficients of UF-1000i test and quantitative microscopy test were 0.994 for WBC and RBC,0.900 for EC and 0.553 for CAST.Conclusion： The performance of UF-1000i urine formed elements analyzer is satisfied.UF-1000i can provide precise results of WBC,RBC and EC,concordance with the reference method,and may be a screening tool for urine formed elements.