摘要
目的检测Glypican-3和Glypican-6在巨大儿胎盘组织中表达,探讨其与巨大儿发病的关系。方法采集216例正常新生儿胎盘组织,153例巨大儿胎盘组织作为研究对象,采用RT-PCR和Western Blot方法,分别从mRNA水平和蛋白水平检测Glypican-3和Glypican-6的表达,统计学分析差异。结果 Glypican-3无论在mRNA水平还是蛋白水平,巨大儿组都稍低于正常新生儿组,两组差别有统计学意义(P<0.05)。Glypican-6在两组间无统计学差异。结论胎盘组织中Glypican-3水平的变化可能与巨大儿的发生有关。
Objective: To measure the expression of Glypican-3 and Glypican-6 in the placenta of macrosomia and investigate their role in the pathogenesis of macrosomia.Methods: MRNA and protein of Glypican-3 and Glypican-6 were measured by RT-PCR and Western blot in placenta tissues of 216 cases of normal birth weight neonate and 153 cases of macrosomia.Differences were statistically compared between these two groups.Results: The expression of Glypican-3 was slightly lower in macrosomia than that of normal birth weight neonate,there were significantly different between them(P0.05).No significance difference of Glypican-6 between the two groups.Conclusion: The changes of expressional levels of Glypican-3 may be related to the occurrence of macrosomia.
出处
《中国优生与遗传杂志》
2013年第1期19-21,共3页
Chinese Journal of Birth Health & Heredity