ROC曲线确立血液学各指标在地贫筛查中的CUT-OFF值

ROC curve establish to cut-off value of hematologic indicators for thalassemia screening

目的用ROC曲线(受试者工作特征曲线)确立各血液学指标在地贫筛查中的切值。方法以SYSMEX五分类血细胞分析仪进行全血细胞计数,记录RBC,HB,MCH,MCV,RDW-CV的数值。用伯乐HPLC进行血红蛋白电泳分析记录HBA2值。所有标本进行地中海贫血基因检测。采用gap-pcr及RDB(反向点杂交)原理对筛查结果进行验证。结果在地贫筛查中MCV和MCH比较有价值,MCV的价值略大于MCH,RDW-CV,和HBA2对β地贫的筛查价值更加的显著,而RBC及HB在地贫筛查中价值不大。结论为减少漏诊及误诊。

Objective： Establish to cut-off value of hematologic indicators for thalassemia screening.Methods： Use SYSMEX hematology analyzer to detect MCV,MCH,RDW-CV,Hb RBC,use HPLC analyzer HBA2.All of examples carry out thalassemia gene test which apply gap-pcr and RDB principle.Result： MCV MCH are better than other hematologic indicators.In thalassemia screening.MCV is higher than MCH RDW-CV HBA2 showed value for β thalassemia screening.RBC and HB have screening of limited value.Conclusion： Laboratory should establish;to suit cutoff value in order to ruduse missed diagnosis and Diagnostic Errors.