摘要
用回流提取法提取新疆药桑桑皮中的总黄酮,采用紫外分光光度法测定桑皮提取液总黄酮含量,并通过单因素和正交试验确定最佳提取工艺条件。研究结果表明提取工艺的最佳优化条件为:回流温度80℃、固液比1∶50、回流时间120min、乙醇体积分数70%vol;该工艺条件下总黄酮的提取率为32.65mg/g;桑皮中总黄酮的提取方法和工艺可行、合理。
The total flavonoids in the tree branch bark of Xinjiang black mulberry （Morus nigra L.） were obtained by reflux extraction method. The total content of flavonoids was measured by UV-spectrophotometric method. The optimal extraction conditions were investigated through single factor and orthogonal experiments. The optimal conditions were as followed： extraction temperature 80℃, solid liquid ratio 1：50, extraction time 120min and ethanol concentration 70%vol. Under the optimal extraction conditions, the extraction efficiency of total flavonoids was 32.65mg/g, which indicated that this method was feasible and reasonable.
出处
《中国酿造》
CAS
2013年第1期57-60,共4页
China Brewing
基金
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BR0905)
关键词
新疆药桑
桑皮
总黄酮
回流提取
Morus nigra L.
mulberry tree bark
total flavonoids
reflux extraction
