双菌混合发酵丢糟生产微生物油脂的研究

Production of microbial oils by dual bacteria mixed fermentation

探索利用假丝酵母和皮状丝孢酵母混合发酵丢糟生产微生物油脂的最佳工艺条件。在对假丝酵母和皮状丝孢进行驯化培养的基础上,设定假丝酵母和皮状丝孢酵母的比例、接种量、培养温度、培养时间为4个因素,进行L(934)正交试验,通过测定油脂含量,获得油脂生产的最佳工艺条件。研究结果表明,双菌发酵丢糟生产微生物油脂的最佳工艺条件:假丝酵母和皮状丝孢酵母的比例为1∶1,接种量为9%,培养温度为32℃,培养时间为4d,在该条件下测得每1000g丢糟发酵物中油脂含量为21.14g。

The optimum conditions were studied on the production of microbial oils co-fermented by Candida and Trichosporon utaneum with distiller＇ s grains. On the basis of domestication to Candida and Trichosporon utaneum, Setting the ratio of Candida and Trichosporon utanemn, the inoculums, the culture temperature and the culture time as four factors, the best process conditions were determined by L9（34） orthogonal test through measuring the content of microbial oils in product. The research shows that the microbial oils content of the distiller＇ s grains co-fermented by Candida and Trichosporon utaneum under the ratio of strains is 2：1, the total in0culum is 9% at 32℃ for 4d was 21.14g per 1000g fermentation products.