黄芪甲苷对异丙肾上腺素诱导心肌梗死保护作用

Protective effect of astragaloside Ⅳ on isoproterenol-induced myocardial infarction in rat

目的研究黄芪甲苷(ASTⅣ)对大剂量异丙肾上腺素(ISO)诱导的大鼠心肌梗死的保护作用。方法皮下注射大剂量ISO建立大鼠心肌梗死动物模型,以心电图ST段抬高值作为心肌缺血指标。行心导管检查,观察不同剂量ASTⅣ对大鼠血流动力学指标的影响;测定血清乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)含量,心肌组织超氧化物歧化酶(SOD)和丙二醛(MDA)水平;并且观察心肌组织形态学改变。结果与模型组比较,不同剂量ASTⅣ均能明显改善大鼠心肌梗死血流动力学指标(P<0.05,P<0.01),降低血清LDH、CK-MB含量(P<0.01),提高心肌组织中SOD活性(P<0.05,P<0.01),并可减少MDA生成(P<0.01),减轻心肌细胞的病理形态学改变。结论 ASTⅣ对ISO诱导的大鼠心肌梗死有保护作用,其机制可能与抗氧化反应有关。

Objective To investigate the protective effect of astragaloside Ⅳ （AST-Ⅳ） on isoproterenol-induced myocardial infarction. Methods Myocardial infarction model of rats was induced by subcutaneous injection of large dose of isoproterenol. Myocardial ischemia was evaluated by electrocardiogram（ECG） ST-segment elevation value. The changes of indexes on hemodynamies in rats were observed by the cardiac catheterization. The levels of lactate dehydrogenase （LDH）, creatine kinase isoenzyme （CK-MB） in blood plasma, the activities of superoxide dismutase （SOD）, and the content of malondialdehyde （MDA） in mycardial tissue were measured. The changes of myocardial morphology were observed as well. Results Compared with the model group, AST Ⅳ could significantly improve the indexes on hemodynamics in myocardial infarction of rats（ P 〈 0.05 or P 〈 0.01 ）, significantly decrease the levels of surum LDH and CK-MB（P 〈0.01 ） ,notably increase the activities of SOD in mycardial tissues（P 〈0.05 or P 〈0. 01 ）, decrease the content of MDA in myocardial tissues（P 〈 0.01 ）, and relieve morphological changes of myocardial cells. Conclusion AST Ⅳ has a protective effect on acute myocardial infarction induced by isoproterenol in rats. The protective mechanisms of AST Ⅳ may be related to anti-oxidation.