金匮肾气丸对肾阳虚小鼠睾丸组织端粒酶活性的促进作用

Acceleration of Jingui Shenqi Pill on the Testis Telomerase Activity in Mice of Shen-yang Deficiency

目的探讨金匮肾气丸对肾阳虚小鼠睾丸组织端粒酶活性的影响。方法以"劳倦过度,房事不节"制备肾阳虚小鼠模型,随机分为模型组及金匮肾气丸治疗组(治疗组),每组15只,另选取15只正常雄鼠作为正常组。正常组常规饲养,每天灌胃蒸馏水0.1mL/10g;模型组造模同时每天灌胃蒸馏水0.1mL/10g;治疗组造模同时每天灌胃金匮肾气丸混悬液0.1mL/10g(浓度0.241g/mL)。共干预4周。4周后采用ELISA法检测各组小鼠睾丸组织端粒酶活性。结果 "劳倦过度、房事不节"法复制肾阳虚模型成功,同时金匮肾气丸对肾阳虚模型小鼠的体征有一定的改善作用。与正常组比较,模型组小鼠睾丸端粒酶活性降低(P<0.01);与模型组比较,治疗组端粒酶活性升高(P<0.01)。结论 "劳倦过度、房事不节"肾阳虚小鼠睾丸端粒酶活性较之正常小鼠明显降低,金匮肾气丸可使肾阳虚小鼠睾丸端粒酶活性得以恢复。

Objective To explore the effects of Jingui Shenqi Pill （JSP） on the testis telomerase activity in mice of Shen yang deficiency syndrome （SYDS）. Methods The SYDS model was prepared in 30 mice by over fatigue and sexual overstrain. They were randomly divided into the model group and the JSP group， 15 in each group. Another 15 normal male mice were selected as the normal group. Mice in the normal group were fed routinely， with distilled water administered intragastrically at the daily dose of 0.1 mL/10 g. Mice in the model group were also administered intragastrically with distilled water at the daily dose of 0.1 mL/10 g while modeling establishment. Mice in the treatment group were administered intragastrically with JSP suspension at 0.1 mL/10 g （the concentration was 0.241 g/mL）. The intervention lasted for 4 weeks. Four weeks later， the testis telomerase activity was detected in the three groups by ELISA. Results The SYDS model was replicated successfully by over fatigue and sexual overstrain. JSP could improve the signs of mice of SYDS. Compared with the normal group， the activity of testis telomerase decreased in the model group （P〈0.01）. Compared with the model group， the testis telomerase activity markedly increased in the treatment group （P〈0.01）. Conclusions The testis telomerase activity in mice of SYDS caused by over fatigue and sexual overstrain obviously decreased， when compared with that in mice of the normal group. JSP could recover its activity.