摘要
目的:分析并探讨绒毛组织中抑癌基因PTEN和抗氧化蛋白PeroxiredoxinⅡ(Prx2)与自然流产的关系。方法:应用RT-PCR法及免疫组织化学SP法检测PTEN和Prx2蛋白及mRNA在30例自然流产患者(实验组)绒毛组织中的表达,并以同期非意愿妊娠要求人工流产的30例正常妊娠患者为对照组。结果:①实验组绒毛组织中PTEN和Prx2蛋白阳性表达率(70.0%,36.7%)明显低于对照组(93.3%,93.4%),差异有统计学意义(P<0.05)。②实验组绒毛组织中PTEN和Prx2 mRNA的表达(0.929±0.100,1.131±0.125)明显低于对照组(0.059±0.010,0.222±0.029),差异有统计学意义(P<0.05)。结论:PTEN和Prx2在自然流产患者绒毛组织中表达明显下降,说明可能与自然流产的发生有一定关系。
Objective： To explore the relation of phosphate and tension homology deleted on chromsome ten （PTEN） and peroxiredoxin Ⅱ （Prx2） to spontaneous abortion. Methods： The expression of PTEN and Prx2 in villi tissue of spontaneous abortion （experimental group, n=30） and unwanted pregnancy （control group, n=30） was detected using immunohistochemistry and RT-PCR. Results： 1）The positive expression of PTEN and Prx2 protein in experimental group （70.0%, 36.7%） was significantly lower than that of the control （93.3%, 93.4%）（P〈0.05）. 2）The expression of PTEN and Prx2 mRNA in experimental group （0.059 ± 0.010, 0.222 ± 0.029） was also significantly lower than that of the control （0.929 ± 0.100, 1.131 ± 0.125）（P〈0.05）. Conclusion： Abnormal expression of PTEN and Prx2 in the villi tissue may have a certain role in the pathophysiology of spontaneous abortion.
出处
《生殖与避孕》
CAS
CSCD
2013年第2期78-82,共5页
Reproduction and Contraception
