卵母细胞固定、染色及压片方法研究

Study of the Fixing, Staining and Tabletting Method of Oocytes

目的:建立卵母细胞(单细胞)固定、染色及压片方法。方法:应用显微操作技术,在体视显微镜下通过液滴法移动、洗涤和抗体孵育卵母细胞。结果:通过点柱法压片的方法成功建立了卵母细胞(单细胞)固定、染色及压片方法。共固定卵母细胞58枚,其中1枚丢失,1枚破损,成功率96.55%。结论:此制片方法解决了卵母细胞在固定、染色及压片中容易丢失和碎裂的问题,从而使卵母细胞和胚胎的实验室研究变得较为简单有效。

Objective： To establish the design and implementation of oocyte 抯 fixing, staining and tabletting method. Method： Adopt liquid droplet displaces, washes and antibody incubates the oocyte under the stereomicroscope, by micromanipulation technology. Result： Oocytes were sucessfully fixed stained and tabletted throug post-pillar fin stopping. A total of 58 oocytes were fixed, tained and tabletted. There were only one lost, the other one ruptured. The rate of failure was 3.45%. Conclusion： This method has successfully solved the prodlem of oocyte loss and fragmentation in the process of cell tablet and will make the laboratory studies of oocyte and embryo become easier and effective.