微小RNA-218在鼻咽癌中的表达及其临床意义

Expression of microRNA-218 in nasopharyngeal carcinoma and its clinical significance

目的:检测微小RNA-218(microRNA-218,miR-218)在鼻咽癌组织中的表达情况,并探讨其临床意义。方法:收集鼻咽癌活检组织标本54例,同时以35例正常及慢性炎性反应鼻咽黏膜活检组织作为对照,采用实时荧光定量PCR(real-time fluorescence quantitative PCR,RFQ-PCR)法检测鼻咽癌组织及对照组织中miR-218的表达情况,分析miR-218表达水平与鼻咽癌临床病理参数之间的关系,以及与患者预后的关系。结果:与对照鼻咽黏膜组织相比,miR-218在鼻咽癌组织中的表达水平普遍下调。鼻咽癌组织中miR-218平均表达量明显低于对照鼻咽上皮组织,差异有统计学意义(P=0.000)。miR-218表达水平在低分化鼻咽癌组织明显低于高/中分化鼻咽癌(P=0.032),临床Ⅲ/Ⅳ期鼻咽癌组织明显低于临床Ⅰ/Ⅱ期鼻咽癌(P=0.007),有转移的鼻咽癌组织明显低于无淋巴结转移的鼻咽癌(P=0.019)。Kaplan-Meier生存分析发现,miR-218表达水平越高,患者生存时间越长(P<0.05)。结论:miR-218与鼻咽癌的发生、临床进展及预后密切相关,它可能成为鼻咽癌新的潜在的治疗靶点及诊断预后分子标志物。

Objective： To （nasopharyngeal carcinoma） tiss obtained from NPC patients an investigate the expression of miR-218 （microRNA-218） in NPC ues and its clinical significance. Methods： A series of 54 tissue biopsies d 35 control tissues with no-NPC （normal nasopharyngeal mucosa or chronic inflammation of nasopharyngeal mucosa） were collected. RFQ-PCR （real-time fluorescence- based quantitative PCR） was used to detect the miR-218 expressions in these samples. The correlations of miR-218 expression with clinicopathological characteristics and prognosis of NPC patients were analyzed. Results： Compared with the control tissues, miR-218 was consistently down-regulated in NPC tissues. The average expression level of miR-218 in NPC tissues was significantly lower than that of the control tissues （P = 0.000）. The expression level of miR-218 in poorly differentiated NPC was significantly reduced as compared with well/moderately differentiated NPC （P = 0.032）. The expression level ofmiR-218 in clinical stagesⅢ/Ⅳ NPCwas lower than that in clinical stages Ⅰ/Ⅱ NPC（P= 0.007）. Moreover, the expression level of miR-218 in NPC patients with lymph node metastasis was significantly lower than that in NPC patients without lymph node metastasis （P = 0.01 9）. NPC patients with a higher level of miR-218 expression had also a longer survival （P 〈 0.05）. Conclusion： The expression ofmiR-218 is associated with carcinogenesis, progression and prognosis of nasopharyngeal carcinoma, so it may be used as a potential therapeutic target, a diagnostic biomarker and a prognostic predictor in patients with NPC in future.