摘要
采用卡宝品红染色压片和石蜡切片法对迪勃纳氏烟草(Nicotiana debneyi)大小孢子发生和雌雄配子体发育过程进行了研究。结果发现:(1)迪勃纳氏烟草为两性花,其小孢子发生和雄配子体发育早于大孢子。(2)迪勃纳氏烟草小孢子发生和发育过程基本正常,减数分裂过程有少数细胞出现滞后染色体、染色体断片和染色体桥等异常现象;其胞质分裂为同时型,四分体为十字交叉型和正四面体型,成熟的花粉粒为2细胞型。(3)迪勃纳氏烟草为2室子房,中轴胎座,倒生胚珠,胚珠多数,胚囊发育为蓼型,大孢子发生和发育过程未观察到异常现象。
The megasporogenesis,microsporogenesis and the development of female and male gametophyte of Nicotiana debneyi were studied by carbol fuchsin staining and paraffin sectioning.The results showed that:(1)N.debneyi is hermaphrodite flower and the microspore develops earlier than megaspore.(2)Microsporogenesis and development of male gametophyte of N.debneyi are normal but there are chromosome bridges,chromosome fragment,laggard chromosome in a few cells.The meiosis in microspore of N.debneyi is simultaneous cytokinesis and the tetards are decussate or tetrahedral types.The mature pollens belong to 2 cell type.(3)N.debneyi is ovary 2-locular,axile placenta,ovules numerous and polygonum type of mature embryo sac.Megasporogenesis and the development of female gametophyte of N.debneyi are normal.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第1期11-16,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
云南省烟草公司项目(2010YN09
2011YN60
2011174)
云南大学生命科学学院植物科学研究所青年教师基金项目(ZW201002)
关键词
迪勃纳氏烟草
大孢子发生
小孢子发生
雌配子体
雄配子体
Nicotiana debneyi
megasporogenesis
microsporogenesis
female gametophyte
male gametophyte