电针对阿尔茨海默病模型大鼠额叶与海马区磷酸化P38丝裂原活化蛋白激酶和白介素-1β mRNA的影响

Effect of Electroacupuncture on Expression of Phosphorylated P 38 MAPK and IL-1β in Frontal Lobe and Hippocampus in Rats with Alzheimer's Disease

目的:探讨P 38丝裂原活化蛋白激酶(MAPK)、白介素(IL)-1β在阿尔茨海默病发病中的作用及电针对其的影响。方法:将SD大鼠随机分成正常组、假手术组、模型组和电针组,每组8只。Meynert核注射微量Aβ1-40制备阿尔茨海默病模型。电针组取"百会""太溪""足三里",每次治疗15min,每天1次,6次1个疗程,间隔1d后再治疗1个疗程。采用Western blot检测额叶与海马区磷酸化P 38MAPK的表达;RT-PCR检测IL-1βmRNA表达。结果:与正常组、假手术组相比,模型组额叶与海马区磷酸化P 38MAPK、IL-1βmRNA表达增强(P<0.01);与模型组相比,电针组上述两指标表达减少(P<0.01,P<0.05)。结论:电针对阿尔茨海默病模型大鼠P 38MAPK磷酸化有调节作用,从而阻断其介导的免疫炎性反应。

Objective To observe the effect of electroacupuncture （EA） intervention on expression of phosphorylated mitogen activated protein kinase-P 38 （p-P 38 MAPK） protein and Interleukin 1β（IL-1β）mRNA in the frontal lobe and hippocampus in Alzheimer＇s disease （AD） rats so as to explore its mechanisms underlying improvement of AD in clinic. Methods Thirty-two SD rats were equally and randomly divided into normal control （normal）, sham-operation （sham）, model and EA groups. AD model was established by microinjection of A_β1-40 （ 10 μg/μL, 1μL） into bilateral Meynert nucleus （AP： 1. 4 mm, L： 3 mm, H：7 mm） and validated by water maze tests. Rats of the sham group were treated by microinjection of the same dose of normal saline into the bilateral Meynert Nucleus. EA （1 mA, 2 Hz） was applied to bilateral ＂Baihui＂ （GV 20）, ＂Taixi＂ （KI 3） and ＂Zusan- li＂ （ST 36） for 15 min, once daily for 12 sessions. Expression levels of p-P38 MAPK protein and IL-1β mRNA in the hippocampus and frontal lobe tissues were detected by Western blot and RT-POR, respectively. Results In comparison with the normal group, the expression levels of p-P 38 MAPK protein and IL-1β mRNA in the hippocampus and frontal lobe tissues were upregulated significantly in the model group （P〈0.01）. After 12 sessions of EA intervention, the expression levels of both p-P 38 MAPK pro- tein and IL-1β mRNA were down-regulated significantly （P〈0. 01, P〈0. 05） in spite of being still higher than those of the normal group. No significant differences were found between the normal and sham groups in the expression levels of both p-P 38 MAPK protein and IL-1β mRNA （P〉0. 05）. Conclusion EA intervention can reduce the over expression of both p-P 38 MAPK protein and IL-1β mRNA in the hippocampus and frontal cortex in AD rats, suggesting an improvement of AD after EA intervention by re- straining the inflammatory reaction.