电针不同部位单穴/穴组对大鼠胃运动调节效应的特征及影响因素

Influence of Electroacupuncture Stimulation of “Tianshu”(ST 25),“Quchi”(LI 11) and “Shangjuxu”(ST 37) and Their Pairs on Gastric Motility in the Rat

目的:观察电针不同部位单穴/穴组对胃运动的影响,探讨不同状态下电针对胃肠运动的调节作用。方法:将SD大鼠随机分为正常组、饥饿组(禁食24h)、阿托品组(M受体拮抗剂,0·1%,0·8mL/kg,40μL·min-1·kg-1)、乙酰胆碱组(M受体激动剂,0·1%,20μL·min-1·kg-1)、克伦特罗组(β2受体激动剂,0·2%,80μL·min-1·kg-1)、心得安组(β受体拮抗剂,0·4%,1mL/kg,40μL·min-1·kg-1)和对穴组,每组30只。在胃内放置球囊,并连接到压力传感器检测胃内压力。分别在上述6种状态下观察电针(2Hz/15Hz,2mA,2min)"天枢""曲池"和"上巨虚"对大鼠胃运动的影响,并观察正常状态下电针"天枢"+"上巨虚""天枢"+"曲池"和"上巨虚"+"曲池"对大鼠胃运动的影响(均取左侧腧穴)。结果:静脉注射阿托品和克伦特罗之后,胃内压力下降(P<0·05);给予乙酰胆碱和心得安,胃内压升高(P<0·05)。在正常、饥饿、给予M受体激动剂/拮抗剂和β受体激动剂/拮抗剂情况下,电针"天枢"对胃内压和胃运动均产生明显抑制作用,抑制程度为:饥饿>正常,乙酰胆碱>阿托品,克伦特罗>心得安;电针"曲池"和"上巨虚"对胃内压和胃运动均产生明显兴奋作用;"天枢"+"上巨虚"对胃运动以抑制为主,"上巨虚"+"曲池"表现为兴奋作用,而"天枢"+"曲池"的抑制或兴奋作用均不明显。结论:电针腹部"天枢"穴对胃运动以抑制为主,而电针位于四肢的"曲池"和"上巨虚"穴对胃运动以兴奋为主,其效应量和机体状态有关,配伍应用可能会产生拮抗效应,改变腧穴效应的方向。

Objective To observe the effect of electroacupuncture （EA） stimulation of different acupoints or acupoint pairs on gastric motility so as to explore their modulation regularities under different conditions. Methods SD rats were random- ly divided into normal control, starvation （food-deprivation for 24 h）, atropine （antagonist for M-receptor）, acetylcholine （Ach, agonist for M-receptor）, propranolol （antagonist for β-receptor） and clenbuterol （agonist for β 2-receptor） and paired-acupoint groups （30 rats/group）. The intragastric pressure was measured via a pressure transducer connected to a balloon inserted in the stomach cavity. EA （2 Hz /15 Hz, 2 mA） was applied to the left ＂Tianshu＂ （ST 25） ,＂Quchi＂ （LI 11 ） and ＂Shangjuxu＂ （ST 37） which were formed in pairs： ST 25-LI 11, ST 25-ST 37 and LI 11-ST 37 for 2 min following intravenous injection of atropine （0. 1%, 0.8 mL/kg, 40 μL . min-1. kg-1 ） ,0. 1% acetylcholine （20 μL -1.min-1. kg-1 ） ,0.2% clenbuterol （80 μL . min-1. kg-1 ） and 0.4% propranolol （1 mL/kg,40 μL.min-1. kg-1） under food-deprivation conditions. Results After intravenous injection of at- ropine and clenbuterol, the intragastric pressure were decreased significantly （P〈0.05）, while after administration of Ach and propranolol, the intragastric pressure increased markedly （P〈0.05）. Under normal and starvation conditions, and after intrave- nous administration of M-receptor antagonist （atropine） and agonist （Ach）, β-receptor antagonist （propranolol） and agonist （clen-buterol）, EA stimulation of ST 25 produced an apparently inhibitory effects on gastric motility （80.00%, 86.67%, 76.67%, 86.67 %, 73.33 % and 86.67 %, respectively） and intragatric pressure （P〈0.05） with the tendency being starvation 〉normal, acetylcholine 〉atropine and clenbuterol 〉propranolol. Whereas EA stimulation of LI 11 and ST 37 mainly produced an excitatory effect on gastric motility （60.00%, 56.67%, 93.33%, 40.00%, 53.33% and 50.00%, respectively for LI 11 ; 66.67%, 60.00%, 80.00%, 53.33%, 46.67% and 73.33%, respectively for ST 37）. Following EA stimulation of the paired-acupoint groups, ST 25-ST 37 induced a predominately inhibitory effect on gastric motility （50.00%） and intragastric pressure, while LI 1 1-ST 37 stimulation had a principally excitatory effect on gastric motility （53.33%）, and ST 25-LI 11 showed no apparent effect （50.00 % ）. CenclusJen EA stimulation of ST 25 area at the abdomen produces a predominant inhibitory effect on gastric motility, while EA of LI 11 and ST 37 on the upper and lower limbs induces an excitatory effect on gastric movement, when applied in pairs, EA of ST 25-ST 37 suppresses the gastric activity, and LI 11-ST 37 promotes the gastric activity, suggesting a specificity of the effect of different acupoint stimulation.