电针干预对大强度耐力运动机体自由基代谢的影响

Influence of Electroacupuncture Intervention on Free Radical Metabolism in Athletes Undergoing Intensive Endurance Exercise

目的:观察电针对大强度耐力运动机体自由基代谢的影响,为电针干预运动性疲劳提供理论和临床依据。方法:将30名男大学生随机分为空白组、训练组和电针组,每组10人,训练组和电针组采用哈佛台阶法进行定量负荷实验,连续运动15d,空白组不采用干预手段。每次运动后电针组采用电针方法恢复,训练组采用单纯休息恢复。训练组和电针组受试者分别于第1天运动前、第1天运动后、电针结束后与空白组同时间采用硫代巴比妥法检测血清丙二醛(MDA)含量和黄嘌呤氧化法检测血清超氧化物歧化酶(SOD)活性。结果:与空白组及运动前比较,训练组和电针组第1天运动后血清MDA含量均显著升高(P<0.05),SOD的活性极显著升高(P<0.01),SOD/MDA比值显著下降(P<0.01);与第1天运动后比较,第15天运动后电针组MDA含量显著下降(P<0.05),训练组SOD活性显著下降(P<0.05);与训练组比较,第15天运动后电针组MDA含量显著下降(P<0.05),SOD活性显著上升(P<0.01),SOD/MDA显著上升(P<0.01)。结论:电针干预能显著降低大强度耐力运动机体血清中MDA含量,增强血清中SOD活性,从而较好地抑制氧自由基对机体的损害,促进运动性疲劳的消除。

Objective To observe the effect of electroacupuncture （EA） intervention on free radical metabolism in ath- letes during intensive endurance exercise, so as to reveal its mechanism underlying fatigue relief. Methods Thirty male college student volunteers were equally divided into control group, intensive endurance exercise group （exercise group for short） and EA group. Student volunteers in both exercise and EA groups were asked to conduct fixed quantitative load exercise （Harvard step test） for 30 min, once daily for 15 days. EA was applied to bilateral Xuehai （SP 10）, Zusanli （ST36）, Taichong （LR3）, and San- yinjiao （SP 6） for 30 min, once daily for 15 days. Serum malondialdehyde （MDA） contents and superoxide dismutase （SOD） ac- tivity levels on the day before exercise, one day after exercise and 15 days after EA intervention were detected by using thioma- Ionylurea colorimetric method and xanthine oxidase method, respectively. Results Compared with pre-exercise or the control group, serum MDA content and SOD activity after one day＇s intensive endurance exercise were increased significantly in both exercise and EA groups （P〈0.05, P〈0.01 ）, and the ratios of SOD/MDA decreased considerably in the latter two groups （P〈0.01）. At the time-point of post-EA intervention, serum MDA level was significantly higher in the exercise group than in the control group （ P〈0.05）, serum MDA content in the EA group was significantly lower than that in the exercise group （ P〈0.05）, and serum SOD activity in the EA group was significantly higher than that in the exercise group （P〈0.01）. The ratio of SOD/ MDA was increased obviously in the EA group than in the exercise group （ P〈0.01 ）. Conclusion EA can decrease serum MDA content and increase SOD activity and SOD/MDA in athletes experiencing intensive endurance exercise, which may contri- bute to its effect in relieving athletic fatigue.