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布鲁氏菌rOmp31_(48-74)-BLS蛋白的多克隆抗体制备与检测 被引量：1

Polyclonal Antibody Preparation and Detection of rOmp31_(48-74)-BLS Peptide from Brucella
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摘要 目的:研究布鲁氏菌Omp31分子的免疫原性。方法:利用IPTG诱导rOmp3148-74-BLS融合重组蛋白表达,经过His-tag镍柱进行亲和纯化之后,作为抗原免疫家兔制备多克隆抗体。结果:rOmp3148-74-BLS重组蛋白能够被大肠杆菌正确表达,而且,能够作为有效的抗原刺激家兔产生可以相互识别的多克隆抗体。结论:Omp31分子具有较好的免疫原性,可以引起较强的免疫反应。 Objective： The immunogenicity of Brucella Omp3 1 molecule was studied. Method： The rOmp3148-74 - BLS fusion protein was induced to express using IPTG. After purified using His -tag nickel affinity column,the rOmp3148-74 -BLS fusion protein was used as the antigen to stimulate the rabbit to produce antibody. Result： The rOmp3148-74 - BLS fusion protein could be correctly expressed by E- . coli. And it could be used as the effective antigen to stimulate rabbit to produce polyclonal antibody. Conclusion：Brucella Omp31 mole- cule possessed good immunogenicity,and could cause a strong immune response.
作者 杜志强 王建英
机构地区 内蒙古科技大学数理与生物工程学院生物工程与技术研究所
出处 《生物技术》 CAS CSCD 北大核心 2012年第5期43-45,共3页 Biotechnology
关键词 布鲁氏菌 bls基因 omp31基因 融合蛋白质 多克隆抗体 Brucella bls gene omp31 gene fusion protein polyclonal antibody
分类号 Q785 [生物学—分子生物学]
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参考文献10

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生物技术
2012年 第5期

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