栽培灯盏花DNA指纹图谱研究及遗传多样性分析

Construction of DNA Fingerprinting and Analysis of Genetic Diversity with ISSR Markers for Erigeron breviscapus

目的:采用ISSR标记构建云南省栽培灯盏花DNA指纹图谱并进行遗传多样性分析。方法:从20对候选引物中筛选出7对引物对云南10个地方栽培灯盏花进行扩增,通过扩增带型的差异构建其DNA指纹图谱,利用Popgen32、NTSYS2软件进行遗传一致性系数和遗传多样分析。结果:7对引物在10份共扩增出48条谱带,其中33条具有多态性,占68.8%,表明各居群的遗传差异较大。10个地方种质资源被聚为2个大类,野生居群被聚为一类,栽培灯盏花聚为一类,其中栽培灯盏花有两大亚类。结论:通过构建DNA指纹图谱容易地把灯盏花种植资源相互区分鉴别出来,10个地方灯盏花种质资源遗传多样性丰富,栽培灯盏花与野生具有差异,栽培种质材料之间也有差异。

Objective：Used ISSR markers to construct DNA fingerprinting and analysis genetic diversity of 10 medical plants Erigeron bre- viscapus major cultivars form Yunnan. Method：Seven ISSR primer pairs with high polymorphisms and good repeatability were successfully screened out from 20 candidates to construct the fingerprinting database. Used Popgcn32, NTSYS2 biological software to analysis of genetic diversity and genetic uniformity coefficient. Result：7 ISSR primer pairs had 48 fragments among the10 varieties,and 33 fragments of the 68. 8% total were polymorphic. It showed the genetic had differences of populations. 10 regional resources were clustered into three groupso Conclusion： Breviseapus planting resources were easily identified out by constructing DNA fingerprint. The genetic diversity was rich in 10 regions Erigeron breviscapus. They have different each other.