黄芪注射液对L_6成肌细胞抗H_2O_2致凋亡模型的保护

Protection of Astragalus Injection on H_2O_2 Apoptosis Model Resistance in L_6 Myoblast Cells

目的:探讨黄芪注射液对H2O2损伤L6大鼠成肌细胞的修复。方法:取1、0.5、0.1、0.05mmol/L H2O2损伤成肌细胞建立模型,再取2 000、1 000、500、250、125、62.5mg/mL黄芪注射液实施保护。应用MTT法、流式细胞仪及荧光抗体Bax和Bcl-2检测,使用SPSS 15.0统计软件处理数据。结果:经0.1mmol/L H2O2损伤成肌细胞存活率由94.4±5.7%降至32.6±3.8%,凋亡率由0增至32.45±2.9%。黄芪注射液处理实验组细胞存活率64.5±4.8～82.2±9.6%较模型组32.6±3.8%增加,凋亡率也从32.45±2.9%减至2.96±0.5～9.29±2.7%,荧光抗体检测Bcl-2细胞数57.7±4.3～74.2±6.9,Bax细胞数42.3±4.0～60.2±5.1。结论:黄芪注射液对大鼠成肌细胞H2O2损伤经由p38MAPK信号通路实施保护。

Objective：Astragalus injection was approached for repair of the L6 rat myoblast cells against H202 injury. Method：The concen- tration of 1,0. 5,0. 1,0. 05retool/L H202 were selected on myoblast cell injury models. The dose of Astragalus injection 2 000,1 000, 500,250,125,62. 5 mg / mL were added to protect damage cultured ceils. MTT method,flow cytometry and fluorescent antibody of bcl -2 and bax were detected. The application of SPSS 15. 0 statistical software was used to analyse data. Result：When myoblast cells was dam- aged by 0. lmmol/L H202 ,the rate of cell survival was reduced from 94. 4 ± 5.7% to 32. 6 ± 3. 8%, and the rate of apoptosis was in- creased from 0 to 32. 45±2. 9%. The survival rate of experiment group treated by Astragalus injection was raised 64. 5 ± 4. 8-82. 2 ±9. 6% with injury model 32. 6 ±3.87% and the rate of apoptosis was decreased from 32.45±2. 9% to 2. 96 ±0. 5 -9. 29 ±2. 7%. Fluo- rescent antibody was detected for Bcl - 2 cell number of 57.7 ±4. 3 - 74. 2 ± 6. 9, Bax cell count 42. 3 ± 4. 0 - 60. 2 ±5. 1. Conclusion： The rat myoblast cells against H202 injury were protected by Astragalus injection via p38MAPK signaling pathway.