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实时荧光定量PCR技术在鱼类病害研究中的应用 被引量:3

Real-time PCR Technology and Its Applications in Fish Diseases
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摘要 实时荧光定量PCR技术是一种新的核酸定量技术,通过检测PCR产物中荧光信号强度达到定量的目的,与常规PCR相比,具有无污染、特异性强、检测灵敏、定量准确等特点,该技术在分子诊断、动植物检疫等方面得到了广泛的应用。目前水产养殖业处于飞速发展时期,其中鱼类的病害问题也日益突出,为了预防和控制鱼类病害,实时荧光定量PCR技术已逐渐应用于鱼类病害的研究中。该文将从实时荧光定量PCR的技术原理、主要类型以及实时荧光定量PCR技术在鱼类病害研究的应用研究作一综述。 Real - time fluorescent quantitative ( Real - time PCR) is a new kind of nucleic acid technology, which can monitor the whole PCR process synchronously through the accumulation of fluorescence signal. The technology make PCR amplification and final product de- tection go on completely in closed condition, thus Real - time PCR is no pollution. On the other hand, it has such advantages as high sensi- bility, specificity and accuracy. So, Real- time PCR technology has been used widely indifferent fields. Currently, with the development of aquaculture, the disease of fish is more and more serious. In order to prevent and control the fish diseases, Real -time PCR technology was also introduced into and applied in fish disease research. This paper covers the development of Real - time PCR, its principle, types and features, furthermore, the application and prospects of Real - time PCR in fish diseases field were reviewed.
作者 隗黎丽
出处 《生物技术》 CAS CSCD 北大核心 2012年第5期82-85,共4页 Biotechnology
基金 江西省科技厅科技计划项目("黄颡鱼重大新流行性疾病‘裂头病’的早期诊断 预警及免疫防治技术研究" 编号:20111BBF60021) 江西省教育厅科技计划项目("草鱼烂鳃病相关基因的筛选及鉴定" 编号:GJJ11087)资助
关键词 实时荧光定量PCR 鱼类 病害研究 应用 Real - time PCR fish disease research applications
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  • 1Ke L D,Chen Z,Yung W K. A reliability test of standard-based quantitative PCB:exogenous vs endogenous standards[J].Molecular and Cellular Probes,2000,(02):127-135.
  • 2Agindotan B O,Shiel P J,Berger P H. Simultaneous detection of potato viruses,PLRV,PVA,PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR[J].Virology Methods,2007.1-9.
  • 3Marisa L W,Juan F M. Real-time PCR for mRNA quantítatíon[J].Biology Techniques,2005,(01):1-11.
  • 4Ramos-Payan R,Aguilar-Medina M,Estrada-Parra S. Quantification of cy-tokine gene expression using an economical real-time polymerase chain reaction method based on SYBR Green Ⅰ[J].Scandinavian Journal Immunology,2003.439-445.
  • 5Bernard P S,Wittwer C T. Homogeneous amplification and variant detection by fluorescent hybridization probes[J].Clinical Chemical,2000.147-148.
  • 6Beruard P S,Ajioka R S,Kushner J P. Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes[J].American Journal of Pathology,1998.1055-1061.
  • 7Lay M J,Wittwer C T. Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR[J].Clinical Chemistry,1997.2262-2267.
  • 8Higuchi R,Fockler C,Dollinger G. Kinetic PCR analysis:real-time monitoring of DNA amplification reactions[J].Bio-Technology,1993.1026-1030.
  • 9Held C A,Stevens J,Livak K J. Real time quantitative PCR[J].Genome Research,1996.986-994.
  • 10徐淑菲,孔繁德,高隆英,刘荭,陈信忠,龚艳青.SYBR-GreenⅠ实时荧光PCR检测传染性鲑鱼贫血病[J].检验检疫科学,2007,17(5):27-31. 被引量:10

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