兔VX2肝癌HIFU治疗前后肿瘤新生血管MSCT灌注成像研究

Neovascularization in survival cancerous tissue after high intensity focused ultrasound therapy on liver tumor using multislice CT perfusion

目的通过对兔肝VX2种植瘤高强度聚焦超声(HIFU)治疗前后MSCT灌注参数与肿瘤微血管密度(MVD)、血管内皮生长因子(VEGF)表达的相关性研究,探讨MSCT灌注成像活体评价肝肿瘤血管生成的价值。方法 30只VX2肝种植瘤模型兔,分为对照组和治疗组,治疗组进行HIFU治疗,分别对两组试验动物行MSCT灌注成像检查,并用免疫组织化学方法测定MVD、VEGF表达情况,分析MSCT灌注参数与MVD和VEGF的相关性。结果治疗组灌注参数、MVD及VEGF表达程度均明显低于对照组,VEGF和MVD的表达呈显著正相关,MSCT灌注参数中,肝总血流灌注量(HBF),毛细血管表面通透性(PS)和肝灌注指数(HAI)与VEGF、MVD的表达均呈高度正相关,而肝血容积(HBV)、平均通过时间(MTT)与VEGF、MVD的表达无相关性。结论 MSCT灌注参数与MVD、VEGF表达有明显相关性,可用于活体评价肿瘤血管生成。

Objective To investigate the correlation of the CT perfusion parameters with microvessel density（MVD） and vascular endothelial growth factor（VEGF） before and after treating by high-intensity focused ultrasound（HIFU） in rabbit VX2 liver tumor model and to explore MSCT perfusion imaging evaluating liver tumor angiogenesis in vivo.Methods Thirty rabbit VX2 liver tumor models were divided into control group and treatment group.The treatment group was given HIFU therapy.Both groups underwent MSCT perfusion scan and the perfusion parameters were obtained and analyzed.Expressions of VEGF and MVD were determined by immunnohistochemical techniques.The correlation of perfusion parameters with MVD and VEGF were analyzed.Results Perfusion parameters,MVD and VEGF in treatment group were significantly lower than those in the control group.There was significant positive correlation between MVD and VEGF,and also between the value of HBF,PS,HAI and MVD,VEGF,while no correlation was observed between the value of MTT,HBV and MVD,VEGF.Conclusion There was significant correlation between perfusion parameters and MVD,VEGF.The perfusion parameters can be used to evaluate tumor angiogenesis in vivo.