Dlx_(1/2)在经Kcl刺激脑皮质神经细胞中的表达研究

Research on Expression of Dlx_(1/2) in Cerebral Cortex Nerve Cells by Kcl Stimulating

目的:观察Dlx1/2在经Kcl刺激大鼠E 17脑皮质神经细胞中的表达,揭示该对基因在核酸与蛋白水平的表达特点及其在大脑接受刺激后反应早期功能活动中的初步作用。方法:原代细胞培养获取SD大鼠E17脑皮质神经细胞;qRT-PCR分析2个基因的核酸在Kcl刺激0～24 h中的表达变化情况;Western blot分析2个基因的蛋白在Kcl刺激0～24 h中的表达变化情况。结果:原代细胞培养显示细胞状态良好。qRT-PCR显示Dlx1/2在经Kcl刺激细胞的24 h内mRNA表达趋势均为先上升后下降,其中6 h是高峰,24 h出现下降。Western blot显示经Kcl刺激后,Dlx1的表达增高只出现在12 h内,24 h出现明显下降;而Dlx2表达随时间延长而明显增高,24 h稍有下降。结论:Dlx1/2的表达与Kcl刺激时间的长短有关,Dlx2表达整体高于Dlx1。Dlx1/2表达升高对胚胎期脑皮质神经细胞早期反应中的活动功能具有重要作用,其中Dlx2的作用可能相对更大。

Objective：To observe the expression of DlX1/2 in 17th days embryonic cerebral cortex nerve cells by Kel stimulating and reveal their expression of nucleic acid and protein level and its initial role in early response activities of function after brain receiving stimulation. Method：To get the cortex nerve cells of 17th days embryonic SD rats by primary cell culture; qRT- PCR and Western blot were performed in researching expression changes during Kcl stimulating from 0 - 24 h. Result： Primary cell culture demonstrated that nerve cells were in good condition, qRT - PCR showed that mRNA expression of Dlxl/2 by Kel stimulating was declined after rising for the first, 6 h was a peak and 24 h was a decrease. Western blot showed that high expression of Dlx1 increasing only occurred within 12 h, 24 h declined obviously after Kcl stimulating;the expression of DLx2 increased with prolongation of stimulation time. Conclusion：The expres- sion of Dlxl/2 was related to the length of stimulation time,then the expression of Dlx1much higher than Dlx2 ＇ s. In embryonic period cere- bral cortex nerve cell＇ s early time response active function, higher expression of the Dlx1/2 has the important regulative func- tion. Particularly Dlx2 function is possible relative more important.