摘要
目的:分析柠檬酸工业废水IC厌氧反应器处理时产生的厌氧颗粒污泥中真细菌的菌群结构。方法:构建细菌的16SrDNA克隆文库,对文库中的16S rDNA基因序列进行测序,然后Blast比对,并进行分类、建系统发育树。结果:对获得的77个16S rDNA序列进行测序,按序列相似性≥97%的分类标准,这些序列可分为22个OTU,其中4个优势OTU分别与棒杆菌属(Corynebacterium)、梭菌属(Clostridium)、消化球菌属(Peptococcus)、疣微菌属(Verrucomicrobia)最为相近,其余OTU的克隆数较少。颗粒污泥中的真细菌主要为放线菌纲(Actinobacteria)、梭菌纲(Clostridia)、拟杆菌纲(Bacteroidetes)以及δ-变形菌纲(Deltaproteobacteria)的细菌,分别占克隆总数的34/77、31/77、6/77、6/77。结论:该文研究了柠檬酸废水处理过程中产生的厌氧颗粒污泥中细菌的菌群组成和结构,为深入了解柠檬酸废水的厌氧处理过程提供了一定的理论借鉴作用。
Objective：To study the bacterial community in the anaerobic Granular Sludge formed during the processing of citric acid indus- trial wastewater. Method： Phylogenetic tree were mapped after the sequences in the 16S rDNA clone library were sequenced and blasted. Result：Clones were placed into operational taxonomic unit （OTU） groups with ≥97% 16S rDNA sequence similarity and 77 se- quences in the 16S rDNA clone library were divided into 22 OTUs after sequencing. The predominant bacterial floras were Corynebacteri- um,Clostridium,Peptococcus, and Verrucomicrobia respectively. The bacterial in the anaerobic Granular Sludge mainly composed of Acti-nobacteria, Clostridia, Bacteroidetes, and Deltaproteobacteria, 34/77, 31/77, 6/77, 6/77 to the total clone number respectively. Conclusion：The study of the bacterial community in anaerobic Granular Sludge provided the theory basis for investigating the citric acid industrial wastewater processing.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第6期60-64,共5页
Biotechnology
