星形胶质细胞上调基因1在喉鳞状细胞癌中的表达及其临床意义

Expression of astrocyte elevated gene-1 protein and its clinical significance in laryngeal squamous cellcarcinoma

目的检测星形胶质细胞上涮基因1（AEG-1）在喉鳞状细胞癌（鳞癌）组织中的表达，并探讨其表达与喉鳞癌临床病理特征和预后之间的关系。方法逆转录聚合酶链反应（RT-PCR）测定AEG-1mRNA在13对配对喉鳞癌及癌旁黏膜组织中的表达。同时，免疫组织化学技术检测AEG-1蛋白在88例喉鳞癌及15例癌旁黏膜组织中的表达，并统计分析其表达水平与临床病理特征及预后的关系。结果AEG-1蛋白在15例癌旁黏膜组织中仅呈阴性或弱阳性表达；AEG-1mRNA在喉鳞癌组织中的相对表达量较癌旁黏膜显著升高（0．81±0．17比0．23±0．10；t=10．337，P〈0．01）；88例喉鳞癌组织中，AEG-1蛋白的阳性表达率为87．5％（77／88）。且AEG-1蛋白表达水平与喉鳞癌患者T分期（X^2=6．289，P=0．018）、临床分期（X^2=11．049，P〈0．01）、转移（X^2=20．859，P〈0．01）及术后复发（X^2=13．459，P〈0．01）密切相关。生存分析显示AEG-1蛋白高表达组与低表达组患者5年总生存率分别为35．9％与86．4％，差异具有统计学意义（X^2=23．409，P〈0．01）；多因素分析示AEG-1蛋白表达水平为喉鳞癌患者预后的独立影响因素（P=0．016）。结论AEG-1蛋白可能在喉鳞癌的发生、发展中发挥了重要作用，对喉鳞癌患者的预后具有评估价值。

Objective To assess the protein expression of astrocyte elevated gene-1 （AEG-1） in tissue specimens of laryngeal squamous cell carcinoma （LSCC）, and to correlate its expression with clinicopathological parameters and prognosis in patients with LSCC. Methods RT-PCR was used to assay the expression of AEG-1 mRNA in 13 pairs of LSCC tissues and their corresponding noncareinoma epithelia. Immunohistochemistry was performed on paraffin-embedded tissue specimens to investigate the protein expression of AEG-I in 88 cases of LSCC specimens and 15 cases of adjacent epithelial samples. Results The expression of AEG-1 mRNA was significantly increased in LSCC tissues compared to adjacent noncarcinoma epithelial tissues （0. 81 ± 0. 17 vs. O. 23 ± 0. 10; t = 10. 337, P 〈 0. 001 ）. Meantime, the positive rate of AEG-1 protein in 88 cases of LSCC was 87.5% （77/88）. However, 15 cases of adjacent noncarcinoma epithelial merely demonstrated negative or mild expression of AEG-1 protein. AEG-I overexpression was closely correlated with T stage （X2 = 6. 289, P = 0. 018 ）, clinical stage （X2 = 11. 049, P〈0.01）, metastasis （X2 =20. 859, P〈0.01）and recurrence（X2 = 13.459, P 〈0.01）. The overall survival rates of patients with AEG-1 overexpression and low expression were 35. 9% and 86. 4%,respectively （X2 23. 409,P 〈 0. 01 ）. Multivariate Cox regression analysis revealed that AEG-1 expression was an independent prognostic factor（P =0. 016）. Conclusion AEG-1 protein may play a critical role in the initiation and progression of LSCC, implicating its predictive value in prognosis.