益气化瘀解毒法对慢性萎缩性胃炎伴异型增生大鼠c-myc的影响

Influence of therapy of tonifying qi, resolving stasis and removing toxicity on c-myc gene in rats with chronic atrophic gastritis complicating dysplasia

目的观察益气化瘀解毒法对慢性萎缩性胃炎伴异型增生模型大鼠c—myc基因的影响。方法将Wistar大鼠60只随机分为造模组50只、空白组10只。造模成功后将造模后剩余的30只大鼠随机分为模型组10只、维酶素组10只、益气化瘀解毒中药组10只。模型组予生理盐水，维酶素组予维酶素悬浊液，益气化瘀解毒中药组予益气化瘀解毒中药制备药液，1次／d。连续治疗12周后用实时荧光定量PCR法及Western blot法对各组大鼠c—myc基因进行检测。结果c—mycmRNA表达量模型组较空白组显著增高（P〈0．01），益气化瘀解毒中药组较模型组显著降低（P〈0．01），益气化瘀解毒中药组较维酶素组也有所降低（P〈0．05），维酶素组较空白组增高（P〈0．05）。益气化瘀解毒中药组与空白组比较差异无统计学意义（P〉0．05），维酶素组和模型组比较差异无统计学意义（P〉0．05）。c—myc蛋白表达量模型组较空白组显著增高（P〈0．01），益气化瘀解毒中药组较模型组显著降低（P〈0．01），维酶素组较模型组也有所降低（P〈0．05），维酶素组较空白组增高（P〈0．05），益气化瘀解毒中药组与空白组比较差异无统计学意义（P〉0．05），益气化瘀解毒中药组和维酶素组比较差异无统计学意义（P〉0．05）。结论中医益气化瘀解毒法可以逆转胃黏膜异型增生，这种作用可能是通过抑制原癌基因c—mycmRNA及c—myc蛋白的表达量而实现的。

Objective To observe the influence of therapy of tonifying qi, resolving stasis and removing toxicity on c-myc gene in rats with chronic atrophic gastritis complicating dysplasia. Methods Wistar rats （n=60） were randomly divided into modeling group （n=50） and blank group （n=10）. After successful modeling the rest of rats （n= 30） were randomly divided into model group, Weimeisu group and Chinese medicinal group （each rt=10）. The model group was give normal saline water, Weimeisu group was given Weimeisu turbid liquid and Chinese medicinal group was given the Chinese medicinals with actions of tonifying qi, resolving stasis and removing toxicity （once a day）. After continuous treatment for 12 weeks, the methods of real-time fluorescence quantitative polymerase chain reaction （RT-PCR） and Western blot were used for detecting c-myc gene in rats of all groups. Results The expression of c-myc mRNA increased significantly in model group compared with blank group （P〈 0.01）, decreased significantly in Chinese medicinal group compared with model group （P〈0.01）, and decreased in Chinese medicinal group compared with Weimeisu group （P〈0.05）, and increased in Weimeisu group compared with blank group （P〈0.05）. The difference in the comparison between Chinese medicinal group and blank group had no statistical significance （P〉0.05）, and that between Weimeisu group and model group had no statistical significance （P〉0.05）. The expression of c-myc protein was significantly higher in model group than that in blank group （P〈0.01）, lower significantly in Chinese medicinal group than that in model group （P〈0.01）, lower in Weimeisu group than that in model group （P〈0.05） and higher in Weimeisu group than that in blank group （P〈 0.05）. The difference in the comparison between Chinese medicinal group and blank group had no statistical significance （P〉0.05）, and that between Chinese medicinal group and Weimeisu group had no statistical significance （P〉0.05）. Conclusion The therapy of tonifying qi, resolving stasis and removing toxicity can reverse gastric mucosal dysplasia through inhibiting the expressions of c-myc mRNA and c-myc protein.