健脾清热活血化瘀中药对慢性萎缩性胃炎伴不典型增生模型大鼠胃黏膜细胞线粒体膜电位的影响

Influence of Chinese medicinals with actions of fortifying the spleen, clearing heat, activating blood and resolving stasis on gastric mucosal mitochondrial membrane potential in rat model of chronic atrophic gastritis accompanied by atypical hyperplasia

目的观察健脾清热活血化瘀中药对慢性萎缩性胃炎伴不典型增生大鼠胃黏膜组织学改变的影响,以探讨中药的作用机制。方法将70只大鼠随机分为正常组、模型组、造模空白组、中药预防组、西药预防组、中药治疗组、西药治疗组。采用化学药物刺激的方法复制了慢性萎缩性胃炎伴不典型增生动物模型,用流式细胞仪检测各组大鼠胃组织中细胞线粒体膜电位的变化。结果线粒体膜电位模型组较正常组升高,差异有统计学意义(P<0.05)。中药预防组、西药预防组均较模型组降低,差异有统计学意义(P<0.05)。中药预防组、西药预防组均较正常组升高,差异有统计学意义(P<0.05)。中药预防组较西药预防组降低,差异有统计学意义(P<0.05)。中药预防组疗效好于西药预防组。线粒体膜电位造模空白组较正常组升高,差异有统计学意义(P<0.05)。中药治疗组、西药治疗组均较造模空白组降低,差异有统计学意义(P<0.05)。西药治疗组较正常组升高,差异有统计学意义(P<0.05),中药治疗组较正常组比较差异无统计学意义(P>0.05),中药治疗组较西药治疗组降低,差异有统计学意义(P<0.05)。中药治疗组疗效好于西药治疗组。结论健脾清热活血化瘀中药可以通过降低胃组织中细胞线粒体膜电位达到对慢性萎缩性胃炎伴不典型增生的治疗作用。

Objective To observe the influence of Chinese medicinals with actions of fortifying the spleen, clearing heat, activating blood and resolving stasis on gastric mucosal histological changes in rat model of chronic atrophic gastritis （CAG） accompanied by atypical hyperplasia for discussing the effective mechanism of Chinese medicinals. Methods All 70 rats were randomly divided into normal group, model group, modeling blank group, prevention group with Chinese medicinals （CMP group）, prevention group with Western medicinals （WMP group）, treatment group with Chinese medicinals （CMT group） and treatment group with Western medicinals （WMT group）. The animal model of chronic atrophic gastritis accompanied by atypical hyperplasia was copied by using chemical stimulus method, and changes of gastric mucosal mitochondrial membrane potential （MMP） were detected by using flow cytometer. Results MMP increased in model group compared with normal group （P〈0.05）, decreased in CMP group and WMP group compared with model group （P〈0.05）. MMP increased in CMP group and WMP group compared with normal group （P〈0.05）. MMP decreased in CMP group compared with WMP group （P〈0.05）. The curative effect was higher in CMP group than that in WMP group. MMP increased in modeling blank group compared with normal group （P〈0.05）, and decreased in CMT group and WMT group compared with modeling blank group （P〈0.05）. MMP increased in WMT group compared with normal group （P〈0.05）. There was no statistical significance between CMT group and normal group （P〉0.05）. MMP decreased in CMT group compared with WMT group （P〈0.05）. The curative effect was higher in CMT group than that in WMT group. Conclusion The Chinese medicinals with actions of fortifying the spleen, clearing heat, activating blood and resolving stasis can treat CAG accompanied by atypical hyperplasia through reducing gastric mucosal MMP.