高脂饮食大鼠脂肪细胞因子的表达和胰岛素抵抗的研究

Expression of Adipocytokine and Mechanism of Insulin Resistance in High-fat Diets Rats

目的该研究旨在建立胰岛素抵抗大鼠模型，观察高脂喂养大鼠脂肪细胞因子的表达，研究胰岛素抵抗的发生机制。方法普通级雄性Wistar大鼠24只，称重后随机分为高脂喂养组、IL-6注射组、正常对照组；6周后所有大鼠禁食12h后称重，测FBG、FINS，并按HOMA公式计算胰岛素抵抗指数（HOMA．IR）；ELISA法测定血清IL-6、脂联素水平。结果与正常对照组相比，高脂喂养组血清IL-6水平明显升高，脂联素水平显著下降（P〈0．05）；IL-6注射组的脂联素水平降低（P〈0．05）；相关分析显示，IL-6与HOMA—IR正相关（r=0．75，P〈0．05），与脂联素负相关（r=0．78，P〈0．01）。结论IL-6参与胰岛素抵抗发生机制，可能至少部分是通过减少脂联素的分泌。

Objective To investigate the mechanism of insulin resistance in high-fat diets rats.Method Twenty-four male Wistar rats were randomly divided into 3 groups after weighting：the first group were fed with high-fat diet,the second group were injected with IL-6,the third group were fed with normal diet as control.After six weeks,FBG and FINS were measured and HOMA-IR was assessed by homeostasis mode assessment.The level of adiponectin and interleukin-6 was detected by ELISA.Results The serum level of IL-6 in high fat diet rats was significantly higher and the level of adiponectin was lower compared with the control group（P 〈 0.05）. Interestingly, the level of adiponectin decreased after injection of IL-6（P 〈 0.05）.Correlation analyze indicated that the level of IL-6 was positively correlated with HOMA-IR（r=0.75,P 〈 0.05）,while negatively correlated with adiponectin（r=-0.78,P 〈 0.01）.Conclusion IL-6 may contribute to one of the mechanisms of insulin resistance by decreasing adiponectin secretion.