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基于Nafion-石墨烯复合物固定甲胎蛋白电化学免疫传感器的研究 被引量:2

An electrochemical immunosensor for alpha fetoprotein based on graphene-nafion nanocomposite
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摘要 该文利用Nation-石墨烯复合物和纳米金固定甲胎蛋白抗体(anti—AFP)。构建了高灵敏的电化学免疫传感器。首先将石墨烯分散在Nation溶液中制得Nation一石墨烯的复合膜,并将其固定在玻碳电极(GCE)表面,通过静电吸附和共价键合作用将硫堇(Thi)和纳米金颗粒(nano—Au)依次固定到Nation-石墨烯复合膜修饰的玻碳电极表面。再通过纳米金单层吸附anti—AFP,最后用牛血清蛋白(BSA)封闭电极上的非特异性吸附位点,从而制得了甲胎蛋白免疫传感器。实验结果表明,该修饰电极对不同浓度的甲胎蛋白(AFP)有很好的响应,其线性范围为0.8~100ng/mL,检出限为O.36ng/mL。 A highly sensitive electrochemical immunosensor for the determination of Alpha Fetoprotein (AFP) was developed based on the muhilayer, comprised of Nafion, grapheme and thionine (Thi). First, the graphene composite was prepared by dispersing grapheme in Nafion solution, and dropped onto the surface of glass carbon electrode to form a stable film. Secondly, the obtained electrode was immersed in Thi solution to absorb the Thi molecules. And then, the nano-Au monofilm was formed by the interaction between the amino groups of Thi and Au. Finally, the prepared electrode was fabricated by anti-AFP and block by BSA. The stepwise fabrication process of the immunosensor was characterized by the cyclic vohammetrys, and experimental results show that the immunosensor have good current response on AFP from 0.8 - 100 ng/mL with a detection limit of 0.36 ng/mL.
出处 《化学传感器》 CAS 2012年第4期16-20,共5页 Chemical Sensors
基金 国家自然科学基金资助项目(21075100)
关键词 电化学免疫传感器 甲胎蛋白 复合物 石墨 NATION 纳米金颗粒 玻碳电极 电极表面 electrochemical immunosensor graphene gold nanoparticles alpha fetoprotein bovine serum albumin
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  • 1Nocoselov K S,Geim A K,Firsov A A. Electric field effect in atomically thin carbon films[J].Science,2004.666-669.
  • 2Meyer J C,Geim A K,Novoselov K S. The structure of suspended graphene sheets[J].Nature,2007.60-63.
  • 3Chen D,Tang L H,Li J H. Graphene-based materials in electrochemistry[J].Chemical Society Reviews,2010,(08):3157-3180.
  • 4Kane C L. Materials Science:Erasing electron mass[J].Nature,2005.168-170.
  • 5Zhou M,Zhai Y M,Dong S J. Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide[J].Analytical Chemistry,2009.5603-5613.
  • 6Yang Z J,Liu H,Zong C. Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay[J].Analytical Chemistry,2009,(13):5484-5489.
  • 7Du D,Zou Z X,Wang J. Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres[J].Analytical Chemistry,2010,(07):2989-2995.
  • 8Frens G. Controlled nucleation for the regulation of the particle size in monodisperse gold suspension[J].Nature,1973.20-22.

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  • 2刘祥萍,黄薇.高效液相色谱法同时测定食品中的甲基香兰素和乙基香兰素[J].中华预防医学杂志,2006,40(4):296-297. 被引量:14
  • 3史贤明,龙飞,崔妍.食源性致病菌快速检测技术研究进展[J].食品科学,2012,Vol.33,(增刊)1:75-80.
  • 4Smith W D.Analytical chemistry at the forefront of homel and defense[J].Anal Chem,2002,74(17):462A-466A.
  • 5Christodoulides N,Tran M,Floriano P N,et al.A Microchip based multi-analyte assay system for the assessment of candiac risk[J].J Anal Chem,2002,74(13):3030-3036.
  • 6Kosuke S,Hiroshi O,Hisashi Y,et al.A reliable and sensitive immunoassay for the determination of crustacean protein in processed foods[J].J Agric Food Chem,2007,55(23):9345-9350.
  • 7Lim S L,Ichinose H,Shinoda T,et al.Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay[J].Anal Chem,2007,79(16):6193--6200.
  • 8Killard A J,Deasy B,O′Kennedy R,et al.Antibodies:production,functions and applications in biosensors[J].TrAC,Trends Anal Chem,1995,14:257-266.
  • 9Faulk W P,Taylor G M.An immunocolloid method for the electron microscope.Immunochemistry[J].Immunochemistry,1971,8(11):1081-1083.
  • 10Guo S J,Dong S J.Biomolecule-nanoparticle Hybrids For Electrochemical Biosensors[J].Trends Anal.Chem,2009,28(1):96-109.

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