银掺杂聚L-甲硫氨酸修饰电极测定6-巯基嘌呤

Silver doped poly(L-methionine) modified glassy carbon electrode for the determination of 6-mercaptopurine

利用循环伏安法制备了银掺杂聚L-甲硫氨酸修饰玻碳电极（Ag—PLM／GCE），研究了6-巯基嘌呤在该修饰电极上的电化学行为，建立了循环伏安法测定6-巯基嘌呤的新方法。在pH6．5磷酸盐缓冲溶液中，扫描速率为140mV／s,6-巯基嘌呤在银掺杂聚L-甲硫氨酸修饰玻碳电极上出现一个氧化峰，峰电位为0．410V。用循环伏安法测定时，其峰电流与6-巯基嘌呤的浓度在3．00×10^-5——5．00×10^-3mol/L范围内呈现良好的线性关系，检出限为5．0×10^-6mol／L。用于乐疾宁片剂中6-巯基嘌呤含量的测定，结果满意。

The silver doped poly（L-methionine） modified glassy carbon electrode was fabricated with cyclic vohammetric method. The electrochemical behavior and determination method of 6-mercaptopurine were studied on the modified electrode. In pH6.5 phosphate buffer solution ,the modified electrode gave a sensitive oxidation peak at 0.410 V （ vs. Ag/AgC1） at the scan rate of 140 mV/s. The linear range for the determination of 6-mereaptopurine was 3.0 0 × 10 -5×5.0 0 × 10 -3 mol/L on the silver doped poly （L -methionine） modified electrode Using cyclic Vohammetrie method. The detection limit was 5.0 × 10-s mol/L. This method was applied to the determination of 6- mercaptopurine in drug samples with satisfactory results.