米诺环素对癫痫海马小胶质细胞P2X7表达的影响

The Effect of Minocycline on The Expression of P2X7 on Hippocampal Microglia in Epileptic Rats

目的:观察米诺环素(minocycline,MC)对癫痫海马小胶质细胞P2X7表达的作用。方法:原代培养大鼠海马小胶质细胞并建立海人酸(kainicacid,KA)损伤模型,并分组:空白对照组、KA对照组、米诺环素处理组,观察各组细胞形态学的变化,检测各组P2X7受体mRNA的表达变化。结果:经KA处理后,海马小胶质细胞从静息状态变为激活状态,而米诺环素处理可以抑制小胶质细胞形态学的改变;经KA处理后,P2X7受体mRNA表达增多,与空白对照组比,升高值为1.825±0.24倍,有统计学意义(P<0.05);而米诺环素处理组,P2X7受体mRNA表达量为1.114±0.09倍,与KA组比较,有明显的统计学意义,P<0.01。结论:米诺环素能有效抑制癫痫海马小胶质细胞的活化,减少P2X7受体的表达。

Objective：To investigate the effect of Minocycline on the expression of P2X7 on hippocampal microglia in epileptic rats. Methods： Hippocampal microglia cells were cultured with kainic acid （KA）. Then We divided the ceUs into groups described as follow randomly： control group, KA group, minocycline group. At the end of the experiment, we observed the morphology changes of the experimental groups by phase contrast electronic microscope, while explored the expression of P2X7 receptor mRNA in different groups by RT-PCR. Results： KA could activate microglia, while minocycline could obviously inhibited this change. After exposed to KA, P2X7 receptor expression increased in microglia cells; while treated with minocycline, the receptor expression upward trend was inhibited, the difference between KA group and Mino group was statistically significant,P〈0.01. Conclusion： Minocycline can effectively inhibit the activation of hippocampal microglia and releasing of P2X7 receptor expression in epileptic rats.