转IPT基因水稻的抗旱性研究

Studies on Drought Tolerance of IPT Transgenic Rice

水稻抗旱性研究是当前水稻研究的热点。采用农杆菌介导法将IPT基因导入籼稻IR64,并获得了254株再生植株,并对其进行PCR检测和Southern杂交分析,确定目的基因已整合到水稻中。选择两个单拷贝的转基因株系和一个野生型株系在开花期对其进行干旱胁迫,在同等水平干旱胁迫条件下,IPT转基因植株的细胞分裂素含量和叶绿素含量明显高于野生型,叶片温度稍微低于野生型,且转基因植株茎叶衰老延缓,这些结果表明IPT基因在抗旱育种及机理研究方面有积极的研究意义。

Drought resistance has become a hot issue in rice scientific research. By Agrobacterium tumefaciens- mediated, IPT gene was transformed to rice IR64 variety. 254 regenerated were obtained, and PCR and Southern blotting analyzsis were carried on them to confirm integration of target gene in the transgenic plant. Two transgenic single copy events and one wild type were treated with drought stress at flowering stage. Under the same drought stress condition, the cytokinins and chlorophyll contents of transgenic plants were obviously higher than that of the wild type. While the leaf temperature was slightly lower than that of the wild type. The stem and leaf senescence of transgenic plant was delayed. These result shows that IPT gene has positive significance on studying drought tolerance breeding and its mechanism.