期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Ⅱ型内含子介导的基因打靶技术及应用前景

Mobile Group Ⅱ Intron Gene Targeting Technology and its Application
下载PDF
导出
摘要 Ⅱ型内含子除具有核酶的特性之外,还可作为一种可移动的元件特异性地转移到基因组序列中,其转移过程需要内含子RNA和内含子编码蛋白质(IEP)来识别靶标位点,内含子已经作为一种新型的基因打靶工具应用于原核生物的基因插入失活和特定的基因敲除,并在真核生物基因组的功能基因组学和基因治疗中展现了广阔的应用前景。 Mobile group II introns are both ribozymes and unique mobile elements for their high sequence-specific, yet readily flexible target site recognition. Both intron RNA and intron-encoded protein （IEP） are involved in target site recognition. Therefore, intron RNA can be a new type of gene targeting for specific gene disruption and knockout technology in prokaryotes. This technology has great potential for revolutionizing fields in eukaryotes, such as functional genomics and gene therapy.
作者 潘阳阳 郎志宏 朱莉 黄大昉
机构地区 中国农业科学院生物技术研究所
出处 《中国农业科技导报》 CAS CSCD 北大核心 2012年第6期54-61,共8页 Journal of Agricultural Science and Technology
基金 国家自然科学基金项目(30970231)资助
关键词 Ⅱ型内含子 基因打靶 RNPs Targetrons group II intron gene targeting RNPs targetrons
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献33

  • 1Lambowitz A M, Belfort M. Introns as mobile genetic elements[J]. Annu. Rev. Biochem. ,1993,62:587 - 622.
  • 2Ferat J L, Michel F. Group lI self-splicing introns in bacteria [ J]. Nature, 1993,364:358 - 361.
  • 3Toro N. Bacteria and archaea group 11 introns: additional mobile genetic elements in the environment [ J ]. Environ. Microbial. ,2003,5:143 - 151.
  • 4Valles Y, Halanych K M, Boore J L. Group Ⅱ introns break new boundaries: presence in a bilaterian's genome [ J ]. PLoS ONE, 2008,3 : el 488.
  • 5Peebles C L, Benatan E J, Jarrell K A, et al.. GroupⅡ intron self-splicing: development of alternative reaction conditions and identification of a predicted intermediate [ J ]. Cold Spring Harb. Symp. Quant. Biol. ,1987,52:223 -232.
  • 6Toro N, Jimenez-Zurdo J I, Garia-Rodriguez F M. Bacterial group Ⅱ introns: not just splicing [ J ]. FEMS Microbiol. Rev. ,2007,31:342 - 358.
  • 7Toot N, Hausner G, Zimmerly S. Coevolution of group H intron RNA structures with their intron-encode dreverse transcriptases [J]. RNA,2001,7 :1142 -52.
  • 8Nargang F E, Bell J B, Stohl L L, et al.. The DNA sequence and genetic organization of a Neurospora mitochondrial plasmid suggest a relationship to introns and mobile elements [ J ]. Cell, 1984,38:441 - 453.
  • 9Zhong J, Karberg M, Lambowitz A M. Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker [ J]. Nucleic Acids Res. , 2003,31 : 1656 - 1664.
  • 10Lambowitz A M, Zimmerly S. Mobile group Ⅱ introns [ J ]. Annu. Rev. Genet. 2004,38:1-35.
中国农业科技导报
2012年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部