河南省几种白粉菌的ITS序列分析

ITS Sequence Analysis of Several Powdery Mildew from Henan Province

采用改良CTAB法提取河南省5种植物白粉菌DNA,扩增其rDNA内转录间隔区(ITS)序列,并进行亲缘关系分析。结果表明:寄生于凤仙、豇豆和菊芋的叉丝单囊壳属白粉菌,其ITS序列聚在一支,遗传距离小,亲缘关系近;寄生在大叶黄杨上的冬青卫矛白粉菌和番茄上的新番茄粉孢菌,其ITS序列聚在另外一支。此分析结果与5种白粉菌的形态学分类结果相一致,说明ITS序列分析技术可以为病原菌的分类鉴定及系统发育等研究提供依据。

The DNA of five powdery mildews from Henan province were extracted by modified CTAB method,and then the rDNA ITS sequences were amplified to analyze the phylogenetic relationship. The result showed that three ITS sequences of Podosphaera section from Impatiens balsamina ;Vigna un- guiculata and Helianthus tuberosus were in one cluster for their small genetic distances and close genetic relationships, and the ITS sequences of Erysiphe sp. from Euonymus japonicus and Odium neolycopersici from tomato were in another cluster, which was consistent with the classification by the morphological characteristics. Therefore, the application of ITS sequences can provide important theoretical basis for pathogen classification and phylogenetic relationship analysis.